|Maintained by INDOT|
|Length||79.291 mi (127.606 km)|
|Length||0.560 mi (0.901 km)|
|South end||KY 1039 near Florence|
|North end||SR 156 near Florence|
|South central segment|
|Length||17.477 mi (28.127 km)|
|South end||US 50 near Milan|
|North end||I-74 near Penntown|
|North central segment|
|Length||16.271 mi (26.186 km)|
|South end||US 52 / SR 1 in Brookville|
|North end||US 27 / SR 44 in Liberty|
|Length||44.983 mi (72.393 km)|
|South end||SR 124 near Pleasant Mills|
| US 33 in Pleasant Mills|
US 224 in Rivare
US 30 near Townley
US 24 near Woodburn
|North end||SR 1 / SR 8 near Newville|
|Counties||Adams, Allen, DeKalb, Franklin, Ripley, Switzerland, Union|
Only the segment of SR 101 that is concurrent with the U.S. Route 33 (US 33) are included as a part of the National Highway System (NHS). The NHS is a network of highways that are identified as being most important for the economy, mobility and defense of the nation. The highway is maintained by the Indiana Department of Transportation (INDOT) like all other State Roads in the state. The department tracks the traffic volumes along all state roads as a part of its maintenance responsibilities using a metric called average annual daily traffic (AADT). This measurement is a calculation of the traffic level along a segment of roadway for any average day of the year. In 2010, INDOT figured that lowest traffic levels were the 710 vehicles and 60 commercial vehicles used the highway daily between SR 124 and US 33. The peak traffic volumes were 5,740 vehicles and 450 commercial vehicles AADT between Sunman and I–74.
The southernmost portion of the route is 0.48 miles (0.77 km) long and connects SR 156 in Switzerland County with Kentucky Route 1039, which connects to Interstate 71 and U.S. Route 42 near the Kentucky Speedway by crossing the Ohio River on the Markland Dam. Before expansion of the Speedway and extension of KY 1039, the route connected to Kentucky Route 1188, which ended just past the Ohio River at U.S. 42.
South central section
The second portion of SR 101 is 17.47 miles (28.12 km) long and is within Ripley County. This sections of SR 101 begins at U.S. Route 50 (US 50) and heads north towards Milan. The road passes through rural farmland with a few houses as a two-lane highway. The road turns towards the northeast just south of SR 48. After SR 48 the highway turns back due north. This section ends at an interchange with Interstate 74 (I–74) in Penntown.
North central section
The third portion of SR 101 is 16.44 miles (26.46 km) long and starts at US 52 and SR 1 in Brookville. SR 101 runs concurrent with SR 1 north and then SR 101 heads northeast, leaving SR 1. The highway curves around Brookville Lake and continues due north towards Liberty. The road crosses into Union County and turns northeast again, ending at an intersection with US 27 and SR 44 in Liberty.
The fourth and final portion is 45.03 miles (72.47 km) long and has its southern terminus at SR 124 in southeastern Adams County and heads north passing through farmland. The road enters Pleasant Mills and has a short concurrency with US 33. North of Pleasant Mills, the road crosses over the St. Marys River. After the river, the road enters Rivare and starts a concurrency with US 224. The concurrency heads west leaving Rivare heading towards Decatur. The concurrency ends when SR 101 turns back north and US 224 continues towards the west. SR 101 crosses into Allen County and has an intersection with US 30 near Townley. After US 30, the road has an all-way stop at old SR 14, now known as Dawkins Road. After Dawkins Road the road crosses the Norfolk Southern Railway. Then the road enters Woodburn and makes a sharp curve heading towards the east, followed by another curve back north. North of Woodburn, the highway has an interchange with US 24 and crosses the Maumee River. After the river, the road makes a sharp curve towards the west, followed by a sharp curve back north. North of the curves the route has an intersection with SR 37 and crosses into DeKalb County, within which it crosses the St. Joseph River. The northern terminus of SR 101 at an intersection with SR 8 and SR 1.
|Gallatin||Warsaw||0.000||0.000||KY 1039 south||Southern terminus of SR 101|
|Ohio River||Markland Locks and Dam|
|Switzerland||Florence||0.560||0.901||SR 156||Northern terminus of the southern section of SR 101|
|Gap in route|
|Ripley||Washington Township||0.561||0.903||US 50 – Versailles, Dillsboro||Southern terminus of the second section of SR 101|
|Milan||6.394||10.290||SR 350 – Osgood, Aurora|
|Franklin Township||11.833||19.043||SR 48 – Napoleon, Lawrenceburg|
|Adams Township||17.432||28.054||SR 46 – Batesville|
|I-74 – Indianapolis, Cincinnati||Exit number 156 on I-74; northern terminus of the second section of SR 101|
|Gap in route|
|Franklin||Brookville||US 52 / SR 1 south – Rushville, Lawrenceburg, Cincinnati||Southern terminus of the third section of SR 101; Southern end of SR 1 concurrency|
|18.038||29.029||SR 1 north||Northern end of SR 1 concurrency|
|Union||Liberty||34.308||55.213||US 27 / SR 44 – Connersville, Richmond, Cincinnati|
|Gap in route|
|Adams||Pleasant Mills||34.309||55.215||SR 124 – Monroe, Willshire||Southern terminus of the northern section of SR 101|
|36.401||58.582||US 33 south – Willshire, OH||Southern end of US 33 concurrency|
|36.574||58.860||US 33 north – Decatur||Northern end of US 33 concurrency|
|Rivare||40.927||65.866||US 224 east - Van Wert||Eastern end of US 224 concurrency|
|41.927||67.475||US 224 west – Decatur||Western end of US 224 concurrency|
|Allen||Townley||54.195||87.218||US 30 – Fort Wayne, New Haven, Van Wert|
|54.459||87.643||Lincoln Highway (Old US 30)|
|US 24 – Fort Wayne, Toledo|
|Halls Corners||70.704||113.787||SR 37 – Fort Wayne, Hicksville|
|DeKalb||Newville||79.291||127.606||SR 1 / SR 8 – Auburn, Butler, Hicksville||Northern terminus of SR 101|
|1.000 mi = 1.609 km; 1.000 km = 0.621 mi|
- Indiana Department of Transportation (July 2016). Reference Post Book (PDF). Indianapolis: Indiana Department of Transportation. Retrieved February 7, 2017.
- National Highway System: Indiana (PDF) (Map). Federal Highway Administration. December 2003. Retrieved July 24, 2012.
- "National Highway System". Federal Highway Administration. August 26, 2010. Retrieved December 19, 2011.
- "INDOT Traffic Zones". Indiana Department of Transportation. 2010. Archived from the original on May 5, 2011. Retrieved July 24, 2012.
- Google (July 23, 2012). "Overview map of the southern section of SR 101" (Map). Google Maps. Google. Retrieved July 23, 2012.
- Indiana Transportation Map (PDF) (Map). Cartography by INDOT. Indiana Department of Transportation. December 2011. Retrieved July 23, 2012.
- Google (July 23, 2012). "Overview map of the south central section of SR 101" (Map). Google Maps. Google. Retrieved July 23, 2012.
- Google (July 23, 2012). "Overview map of the north central section of SR 101" (Map). Google Maps. Google. Retrieved July 23, 2012.
- Indiana Railroad Map (PDF) (Map). Cartography by INDOT. Indiana Department of Transportation. August 23, 2011. Retrieved July 23, 2012.
- Google (July 23, 2012). "Overview map of the northern section of SR 101" (Map). Google Maps. Google. Retrieved July 23, 2012.