|Description||Around 50–100 million vertebrate animals are used in experiments annually.|
|Subjects||Animal testing, science, medicine, animal welfare, animal rights, ethics|
Animal testing, also known as animal experimentation, animal research and in vivo testing, is the use of non-human animals in experiments that seek to control the variables that affect the behavior or biological system under study. This approach can be contrasted with field studies in which animals are observed in their natural environments or habitats. Experimental research with animals is usually conducted in universities, medical schools, pharmaceutical companies, defense establishments and commercial facilities that provide animal-testing services to industry. The focus of animal testing varies on a continuum from pure research, focusing on developing fundamental knowledge of an organism, to applied research, which may focus on answering some question of great practical importance, such as finding a cure for a disease. Examples of applied research include testing disease treatments, breeding, defense research and toxicology, including cosmetics testing. In education, animal testing is sometimes a component of biology or psychology courses. The practice is regulated to varying degrees in different countries.
It is estimated that the annual use of vertebrate animals—from zebrafish to non-human primates—ranges from tens to more than 100 million. In the European Union, vertebrate species represent 93% of animals used in research, and 11.5 million animals were used there in 2011. By one estimate the number of mice and rats used in the United States alone in 2001 was 80 million. Mice, rats, fish, amphibians and reptiles together account for over 85% of research animals.
Most animals are euthanized after being used in an experiment. Sources of laboratory animals vary between countries and species; most animals are purpose-bred, while a minority are caught in the wild or supplied by dealers who obtain them from auctions and pounds. Supporters of the use of animals in experiments, such as the British Royal Society, argue that virtually every medical achievement in the 20th century relied on the use of animals in some way. The Institute for Laboratory Animal Research of the United States National Academy of Sciences has argued that animal research cannot be replaced by even sophisticated computer models, which are unable to deal with the extremely complex interactions between molecules, cells, tissues, organs, organisms and the environment. Animal rights organizations—such as PETA and BUAV—question the need for and legitimacy of animal testing, arguing that it is cruel and poorly regulated, that medical progress is actually held back by misleading animal models that cannot reliably predict effects in humans, that some of the tests are outdated, that the costs outweigh the benefits, or that animals have the intrinsic right not to be used or harmed in experimentation.
The terms animal testing, animal experimentation, animal research, in vivo testing, and vivisection have similar denotations but different connotations. Literally, "vivisection" means "live sectioning" of an animal, and historically referred only to experiments that involved the dissection of live animals. The term is occasionally used to refer pejoratively to any experiment using living animals; for example, the Encyclopædia Britannica defines "vivisection" as: "Operation on a living animal for experimental rather than healing purposes; more broadly, all experimentation on live animals", although dictionaries point out that the broader definition is "used only by people who are opposed to such work". The word has a negative connotation, implying torture, suffering, and death. The word "vivisection" is preferred by those opposed to this research, whereas scientists typically use the term "animal experimentation".
The earliest references to animal testing are found in the writings of the Greeks in the 2nd and 4th centuries BC. Aristotle and Erasistratus were among the first to perform experiments on living animals. Galen, a 2nd-century Roman physician, dissected pigs and goats; he is known as the "father of vivisection". Avenzoar, a 12th-century Arabic physician in Moorish Spain also practiced dissection; he introduced animal testing as an experimental method of testing surgical procedures before applying them to human patients.
Animals have repeatedly been used throughout the history of biomedical research. In 1831, the founders of the Dublin Zoo were members of the medical profession who were interested in studying animals while they were alive and when they were dead. In the 1880s, Louis Pasteur convincingly demonstrated the germ theory of medicine by inducing anthrax in sheep. In the 1880s, Robert Koch infected mice and guinea pigs with anthrax and tuberculosis. In the 1890s, Ivan Pavlov famously used dogs to describe classical conditioning. In World War I, German agents infected sheep bound for Russia with anthrax, and inoculated mules and horses of the French cavalry with the equine glanders disease. Between 1917 and 1918, the Germans infected mules in Argentina bound for American forces, resulting in the death of 200 mules. Insulin was first isolated from dogs in 1922, and revolutionized the treatment of diabetes. On 3 November 1957, a Soviet dog, Laika, became the first of many animals to orbit the earth. In the 1970s, antibiotic treatments and vaccines for leprosy were developed using armadillos, then given to humans. The ability of humans to change the genetics of animals took a large step forwards in 1974 when Rudolf Jaenisch was able to produce the first transgenic mammal, by integrating DNA from the SV40 virus into the genome of mice. This genetic research progressed rapidly and, in 1996, Dolly the sheep was born, the first mammal to be cloned from an adult cell.
Toxicology testing became important in the 20th century. In the 19th century, laws regulating drugs were more relaxed. For example, in the US, the government could only ban a drug after a company had been prosecuted for selling products that harmed customers. However, in response to the Elixir Sulfanilamide disaster of 1937 in which the eponymous drug killed more than 100 users, the US Congress passed laws that required safety testing of drugs on animals before they could be marketed. Other countries enacted similar legislation. In the 1960s, in reaction to the Thalidomide tragedy, further laws were passed requiring safety testing on pregnant animals before a drug can be sold.
As the experimentation on animals increased, especially the practice of vivisection, so did criticism and controversy. In 1655, the advocate of Galenic physiology Edmund O'Meara said that "the miserable torture of vivisection places the body in an unnatural state". O'Meara and others argued that animal physiology could be affected by pain during vivisection, rendering results unreliable. There were also objections on an ethical basis, contending that the benefit to humans did not justify the harm to animals. Early objections to animal testing also came from another angle—many people believed that animals were inferior to humans and so different that results from animals could not be applied to humans.
On the other side of the debate, those in favor of animal testing held that experiments on animals were necessary to advance medical and biological knowledge. Claude Bernard—who is sometimes known as the "prince of vivisectors" and the father of physiology, and whose wife, Marie Françoise Martin, founded the first anti-vivisection society in France in 1883—famously wrote in 1865 that "the science of life is a superb and dazzlingly lighted hall which may be reached only by passing through a long and ghastly kitchen". Arguing that "experiments on animals [. . .] are entirely conclusive for the toxicology and hygiene of man [. . . T]he effects of these substances are the same on man as on animals, save for differences in degree", Bernard established animal experimentation as part of the standard scientific method.
In 1896, the physiologist and physician Dr. Walter B. Cannon said "The antivivisectionists are the second of the two types Theodore Roosevelt described when he said, 'Common sense without conscience may lead to crime, but conscience without common sense may lead to folly, which is the handmaiden of crime.'" These divisions between pro- and anti-animal testing groups first came to public attention during the Brown Dog affair in the early 1900s, when hundreds of medical students clashed with anti-vivisectionists and police over a memorial to a vivisected dog.
In 1822, the first animal protection law was enacted in the British parliament, followed by the Cruelty to Animals Act (1876), the first law specifically aimed at regulating animal testing. The legislation was promoted by Charles Darwin, who wrote to Ray Lankester in March 1871: "You ask about my opinion on vivisection. I quite agree that it is justifiable for real investigations on physiology; but not for mere damnable and detestable curiosity. It is a subject which makes me sick with horror, so I will not say another word about it, else I shall not sleep to-night." In response to the lobbying by anti-vivisectionists, several organizations were set up in Britain to defend animal research: The Physiological Society was formed in 1876 to give physiologists "mutual benefit and protection", the Association for the Advancement of Medicine by Research was formed in 1882 and focused on policy-making, and the Research Defence Society (now Understanding Animal Research) was formed in 1908 "to make known the facts as to experiments on animals in this country; the immense importance to the welfare of mankind of such experiments and the great saving of human life and health directly attributable to them".
Opposition to the use of animals in medical research first arose in the United States during the 1860s, when Henry Bergh founded the American Society for the Prevention of Cruelty to Animals (ASPCA), with America's first specifically anti-vivisection organization being the American AntiVivisection Society (AAVS), founded in 1883. Antivivisectionists of the era generally believed the spread of mercy was the great cause of civilization, and vivisection was cruel. However, in the USA the antivivisectionists' efforts were defeated in every legislature, overwhelmed by the superior organization and influence of the medical community. Overall, this movement had little legislative success until the passing of the Laboratory Animal Welfare Act, in 1966.
Care and use of animalsEdit
Regulations and lawsEdit
The regulations that apply to animals in laboratories vary across species. In the U.S., under the provisions of the Animal Welfare Act and the Guide for the Care and Use of Laboratory Animals (the Guide), published by the National Academy of Sciences, any procedure can be performed on an animal if it can be successfully argued that it is scientifically justified. In general, researchers are required to consult with the institution's veterinarian and its Institutional Animal Care and Use Committee (IACUC), which every research facility is obliged to maintain. The IACUC must ensure that alternatives, including non-animal alternatives, have been considered, that the experiments are not unnecessarily duplicative, and that pain relief is given unless it would interfere with the study. The IACUCs regulate all vertebrates in testing at institutions receiving federal funds in the USA. Although the provisions of the Animal Welfare Act do not include purpose-bred rodents and birds, these species are equally regulated under Public Health Service policies that govern the IACUCs. The Public Health Service policy oversees the Food and Drug Administration (FDA) and the Centers for Disease Control and Prevention (CDC). The CDC conducts infectious disease research on nonhuman primates, rabbits, mice, and other animals, while FDA requirements cover use of animals in pharmaceutical research. Animal Welfare Act (AWA) regulations are enforced by the USDA, whereas Public Health Service regulations are enforced by OLAW and in many cases by AAALAC.
According to the 2014 U.S. Department of Agriculture Office of the Inspector General (OIG) report—which looked at the oversight of animal use during a three-year period—"some Institutional Animal Care and Use Committees ...did not adequately approve, monitor, or report on experimental procedures on animals". The OIG found that "as a result, animals are not always receiving basic humane care and treatment and, in some cases, pain and distress are not minimized during and after experimental procedures". According to the report, within a three-year period, nearly half of all American laboratories with regulated species were cited for AWA violations relating to improper IACUC oversight. The USDA OIG made similar findings in a 2005 report. With only a broad number of 120 inspectors, the United States Department of Agriculture (USDA) oversees more than 12,000 facilities involved in research, exhibition, breeding, or dealing of animals. Others have criticized the composition of IACUCs, asserting that the committees are predominantly made up of animal researchers and university representatives who may be biased against animal welfare concerns.
Larry Carbone, a laboratory animal veterinarian, writes that, in his experience, IACUCs take their work very seriously regardless of the species involved, though the use of non-human primates always raises what he calls a "red flag of special concern". A study published in Science magazine in July 2001 confirmed the low reliability of IACUC reviews of animal experiments. Funded by the National Science Foundation, the three-year study found that animal-use committees that do not know the specifics of the university and personnel do not make the same approval decisions as those made by animal-use committees that do know the university and personnel. Specifically, blinded committees more often ask for more information rather than approving studies.
Accurate global figures for animal testing are difficult to obtain; it has been estimated that 100 million vertebrates are experimented on around the world every year, 10–11 million of them in the EU. The Nuffield Council on Bioethics reports that global annual estimates range from 50 to 100 million animals. None of the figures include invertebrates such as shrimp and fruit flies.
The USDA/APHIS has published the 2016 animal research statistics. Overall, the number of animals (covered by the Animal Welfare Act) used in research in the US rose 6.9% from 767,622 (2015) to 820,812 (2016). This includes both public and private institutions. By comparing with EU data, where all vertebrate species are counted, Speaking of Research estimated that around 12 million vertebrates were used in research in the US in 2016. A 2015 article published in the Journal of Medical Ethics, argued that the use of animals in the US has dramatically increased in recent years. Researchers found this increase is largely the result of an increased reliance on genetically modified mice in animal studies.
In 1995, researchers at Tufts University Center for Animals and Public Policy estimated that 14–21 million animals were used in American laboratories in 1992, a reduction from a high of 50 million used in 1970. In 1986, the U.S. Congress Office of Technology Assessment reported that estimates of the animals used in the U.S. range from 10 million to upwards of 100 million each year, and that their own best estimate was at least 17 million to 22 million. In 2016, the Department of Agriculture listed 60,979 dogs, 18,898 cats, 71,188 non-human primates, 183,237 guinea pigs, 102,633 hamsters, 139,391 rabbits, 83,059 farm animals, and 161,467 other mammals, a total of 820,812, a figure that includes all mammals except purpose-bred mice and rats. The use of dogs and cats in research in the U.S. decreased from 1973 to 2016 from 195,157 to 60,979, and from 66,165 to 18,898, respectively.
In the UK, Home Office figures show that 3.79 million procedures were carried out in 2017. 2,960 procedures used non-human primates, down over 50% since 1988. A "procedure" refers here to an experiment that might last minutes, several months, or years. Most animals are used in only one procedure: animals are frequently euthanized after the experiment; however death is the endpoint of some procedures. The procedures conducted on animals in the UK in 2017 were categorised as –
- 43% (1.61 million) were assessed as sub-threshold
- 4% (0.14 million) were assessed as non-recovery
- 36% (1.35 million) were assessed as mild
- 15% (0.55 million) were assessed as moderate
- 4% (0.14 million) were assessed as severe
A 'severe' procedure would be, for instance, any test where death is the end-point or fatalities are expected, whereas a 'mild' procedure would be something like a blood test or an MRI scan.
The Three RsEdit
The Three Rs (3Rs) are guiding principles for more ethical use of animals in testing. These were first described by W.M.S. Russell and R.L. Burch in 1959. The 3Rs state:
- Replacement which refers to the preferred use of non-animal methods over animal methods whenever it is possible to achieve the same scientific aims. These methods include computer modeling.
- Reduction which refers to methods that enable researchers to obtain comparable levels of information from fewer animals, or to obtain more information from the same number of animals.
- Refinement which refers to methods that alleviate or minimize potential pain, suffering or distress, and enhance animal welfare for the animals used. These methods include non-invasive techniques.
The 3Rs have a broader scope than simply encouraging alternatives to animal testing, but aim to improve animal welfare and scientific quality where the use of animals can not be avoided. These 3Rs are now implemented in many testing establishments worldwide and have been adopted by various pieces of legislation and regulations.
Despite the widespread acceptance of the 3Rs, many countries—including Canada, Australia, Israel, South Korea, and Germany—have reported rising experimental use of animals in recent years with increased use of mice and, in some cases, fish while reporting declines in the use of cats, dogs, primates, rabbits, guinea pigs, and hamsters. Along with other countries, China has also escalated its use of GM animals, resulting in an increase in overall animal use.[excessive citations]
Although many more invertebrates than vertebrates are used in animal testing, these studies are largely unregulated by law. The most frequently used invertebrate species are Drosophila melanogaster, a fruit fly, and Caenorhabditis elegans, a nematode worm. In the case of C. elegans, the worm's body is completely transparent and the precise lineage of all the organism's cells is known, while studies in the fly D. melanogaster can use an amazing array of genetic tools. These invertebrates offer some advantages over vertebrates in animal testing, including their short life cycle and the ease with which large numbers may be housed and studied. However, the lack of an adaptive immune system and their simple organs prevent worms from being used in several aspects of medical research such as vaccine development. Similarly, the fruit fly immune system differs greatly from that of humans, and diseases in insects can be different from diseases in vertebrates; however, fruit flies and waxworms can be useful in studies to identify novel virulence factors or pharmacologically active compounds.
Several invertebrate systems are considered acceptable alternatives to vertebrates in early-stage discovery screens. Because of similarities between the innate immune system of insects and mammals, insects can replace mammals in some types of studies. Drosophila melanogaster and the Galleria mellonella waxworm have been particularly important for analysis of virulence traits of mammalian pathogens. Waxworms and other insects have also proven valuable for the identification of pharmaceutical compounds with favorable bioavailability. The decision to adopt such models generally involves accepting a lower degree of biological similarity with mammals for significant gains in experimental throughput.
In the U.S., the numbers of rats and mice used is estimated to be from 11 million to between 20 and 100 million a year. Other rodents commonly used are guinea pigs, hamsters, and gerbils. Mice are the most commonly used vertebrate species because of their size, low cost, ease of handling, and fast reproduction rate. Mice are widely considered to be the best model of inherited human disease and share 95% of their genes with humans. With the advent of genetic engineering technology, genetically modified mice can be generated to order and can provide models for a range of human diseases. Rats are also widely used for physiology, toxicology and cancer research, but genetic manipulation is much harder in rats than in mice, which limits the use of these rodents in basic science.
Over 500,000 fish and 9,000 amphibians were used in the UK in 2016. The main species used is the zebrafish, Danio rerio, which are translucent during their embryonic stage, and the African clawed frog, Xenopus laevis. Over 20,000 rabbits were used for animal testing in the UK in 2004. Albino rabbits are used in eye irritancy tests (Draize test) because rabbits have less tear flow than other animals, and the lack of eye pigment in albinos make the effects easier to visualize. The numbers of rabbits used for this purpose has fallen substantially over the past two decades. In 1996, there were 3,693 procedures on rabbits for eye irritation in the UK, and in 2017 this number was just 63. Rabbits are also frequently used for the production of polyclonal antibodies.
Cats are most commonly used in neurological research. In 2016, 18,898 cats were used in the United States alone, around a third of which were used in experiments which have the potential to cause "pain and/or distress" though only 0.1% of cat experiments involved potential pain which was not relieved by anesthetics/analgesics. In the UK, just 198 procedures were carried out on cats in 2017. The number has been around 200 for most of the last decade.
Dogs are widely used in biomedical research, testing, and education—particularly beagles, because they are gentle and easy to handle, and to allow for comparisons with historical data from beagles (a Reduction technique).(citation needed) They are used as models for human and veterinary diseases in cardiology, endocrinology, and bone and joint studies, research that tends to be highly invasive, according to the Humane Society of the United States. The most common use of dogs is in the safety assessment of new medicines for human or veterinary use as a second species following testing in rodents, in accordance with the regulations set out in the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. One of the most significant advancements in medical science involves the use of dogs in developing the answers to insulin production in the body for diabetics and the role of the pancreas in this process. They found that the pancreas was responsible for producing insulin in the body and that removal of the pancreas, resulted in the development of diabetes in the dog. After re-injecting the pancreatic extract, (insulin), the blood glucose levels were significantly lowered. The advancements made in this research involving the use of dogs has resulted in a definite improvement in the quality of life for both humans and animals.
The U.S. Department of Agriculture's Animal Welfare Report shows that 60,979 dogs were used in USDA-registered facilities in 2016. In the UK, according to the UK Home Office, there were 3,847 procedures on dogs in 2017. Of the other large EU users of dogs, Germany conducted 3,976 procedures on dogs in 2016 and France conducted 4,204 procedures in 2016. In both cases this represents under 0.2% of the total number of procedures conducted on animals in the respective countries.
Non-human primates (NHPs) are used in toxicology tests, studies of AIDS and hepatitis, studies of neurology, behavior and cognition, reproduction, genetics, and xenotransplantation. They are caught in the wild or purpose-bred. In the United States and China, most primates are domestically purpose-bred, whereas in Europe the majority are imported purpose-bred. The European Commission reported that in 2011, 6,012 monkeys were experimented on in European laboratories. According to the U.S. Department of Agriculture, there were 71,188 monkeys in U.S. laboratories in 2016. 23,465 monkeys were imported into the U.S. in 2014 including 929 who were caught in the wild. Most of the NHPs used in experiments are macaques; but marmosets, spider monkeys, and squirrel monkeys are also used, and baboons and chimpanzees are used in the US. As of 2015[update], there are approximately 730 chimpanzees in U.S. laboratories.
In a survey in 2003, it was found that 89% of singly-housed primates exhibited self-injurious or abnormal stereotypyical behaviors including pacing, rocking, hair pulling, and biting among others.
The first transgenic primate was produced in 2001, with the development of a method that could introduce new genes into a rhesus macaque. This transgenic technology is now being applied in the search for a treatment for the genetic disorder Huntington's disease. Notable studies on non-human primates have been part of the polio vaccine development, and development of Deep Brain Stimulation, and their current heaviest non-toxicological use occurs in the monkey AIDS model, SIV. In 2008 a proposal to ban all primates experiments in the EU has sparked a vigorous debate.
Animals used by laboratories are largely supplied by specialist dealers. Sources differ for vertebrate and invertebrate animals. Most laboratories breed and raise flies and worms themselves, using strains and mutants supplied from a few main stock centers. For vertebrates, sources include breeders and dealers like Covance and Charles River Laboratories who supply purpose-bred and wild-caught animals; businesses that trade in wild animals such as Nafovanny; and dealers who supply animals sourced from pounds, auctions, and newspaper ads. Animal shelters also supply the laboratories directly. Large centers also exist to distribute strains of genetically modified animals; the International Knockout Mouse Consortium, for example, aims to provide knockout mice for every gene in the mouse genome.
In the U.S., Class A breeders are licensed by the U.S. Department of Agriculture (USDA) to sell animals for research purposes, while Class B dealers are licensed to buy animals from "random sources" such as auctions, pound seizure, and newspaper ads. Some Class B dealers have been accused of kidnapping pets and illegally trapping strays, a practice known as bunching. It was in part out of public concern over the sale of pets to research facilities that the 1966 Laboratory Animal Welfare Act was ushered in—the Senate Committee on Commerce reported in 1966 that stolen pets had been retrieved from Veterans Administration facilities, the Mayo Institute, the University of Pennsylvania, Stanford University, and Harvard and Yale Medical Schools. The USDA recovered at least a dozen stolen pets during a raid on a Class B dealer in Arkansas in 2003.
Four states in the U.S.—Minnesota, Utah, Oklahoma, and Iowa—require their shelters to provide animals to research facilities. Fourteen states explicitly prohibit the practice, while the remainder either allow it or have no relevant legislation.
In the European Union, animal sources are governed by Council Directive 86/609/EEC, which requires lab animals to be specially bred, unless the animal has been lawfully imported and is not a wild animal or a stray. The latter requirement may also be exempted by special arrangement. In 2010 the Directive was revised with EU Directive 2010/63/EU. In the UK, most animals used in experiments are bred for the purpose under the 1988 Animal Protection Act, but wild-caught primates may be used if exceptional and specific justification can be established. The United States also allows the use of wild-caught primates; between 1995 and 1999, 1,580 wild baboons were imported into the U.S. Over half the primates imported between 1995 and 2000 were handled by Charles River Laboratories, or by Covance, which is the single largest importer of primates into the U.S.
Pain and sufferingEdit
According to the USDA, in 2016 501,560 animals (61%) (not including rats, mice, birds, or invertebrates) were used in procedures that did not include more than momentary pain or distress. 247,882 (31%) animals were used in procedures in which pain or distress was relieved by anesthesia, while 71,370 (9%) were used in studies that would cause pain or distress that would not be relieved.
Since 2014, in the UK, every research procedure was retrospectively assessed for severity. The five categories are "sub-threshold", "mild", "moderate", "severe" and "non-recovery", the latter being procedures in which an animal is anesthetized and subsequently killed without recovering consciousness. In 2017, 43% (1.61 million) were assessed as sub-threshold, 4% (0.14 million) were assessed as non-recovery, 36% (1.35 million) were assessed as mild, 15% (0.55 million) were assessed as moderate and 4% (0.14 million) were assessed as severe.
The idea that animals might not feel pain as human beings feel it traces back to the 17th-century French philosopher, René Descartes, who argued that animals do not experience pain and suffering because they lack consciousness. Bernard Rollin of Colorado State University, the principal author of two U.S. federal laws regulating pain relief for animals, writes that researchers remained unsure into the 1980s as to whether animals experience pain, and that veterinarians trained in the U.S. before 1989 were simply taught to ignore animal pain. In his interactions with scientists and other veterinarians, he was regularly asked to "prove" that animals are conscious, and to provide "scientifically acceptable" grounds for claiming that they feel pain. Carbone writes that the view that animals feel pain differently is now a minority view. Academic reviews of the topic are more equivocal, noting that although the argument that animals have at least simple conscious thoughts and feelings has strong support, some critics continue to question how reliably animal mental states can be determined. However, some canine experts are stating that, while intelligence does differ animal to animal, dogs have the intelligence of a two to two-and-a-half-year old. This does support the idea that dogs, at the very least, have some form of consciousness. The ability of invertebrates to experience pain and suffering is less clear, however, legislation in several countries (e.g. U.K., New Zealand, Norway) protects some invertebrate species if they are being used in animal testing.
In the U.S., the defining text on animal welfare regulation in animal testing is the Guide for the Care and Use of Laboratory Animals. This defines the parameters that govern animal testing in the U.S. It states "The ability to experience and respond to pain is widespread in the animal kingdom...Pain is a stressor and, if not relieved, can lead to unacceptable levels of stress and distress in animals." The Guide states that the ability to recognize the symptoms of pain in different species is vital in efficiently applying pain relief and that it is essential for the people caring for and using animals to be entirely familiar with these symptoms. On the subject of analgesics used to relieve pain, the Guide states "The selection of the most appropriate analgesic or anesthetic should reflect professional judgment as to which best meets clinical and humane requirements without compromising the scientific aspects of the research protocol". Accordingly, all issues of animal pain and distress, and their potential treatment with analgesia and anesthesia, are required regulatory issues in receiving animal protocol approval.
In 2019, Katrien Devolder and Matthias Eggel proposed gene editing research animals to remove the ability to feel pain. This would be an intermediate step towards eventually stopping all experimentation on animals and adopting alternatives. Additionally, this would not stop research animals from experiencing psychological harm.
Regulations require that scientists use as few animals as possible, especially for terminal experiments. However, while policy makers consider suffering to be the central issue and see animal euthanasia as a way to reduce suffering, others, such as the RSPCA, argue that the lives of laboratory animals have intrinsic value. Regulations focus on whether particular methods cause pain and suffering, not whether their death is undesirable in itself. The animals are euthanized at the end of studies for sample collection or post-mortem examination; during studies if their pain or suffering falls into certain categories regarded as unacceptable, such as depression, infection that is unresponsive to treatment, or the failure of large animals to eat for five days; or when they are unsuitable for breeding or unwanted for some other reason.
Methods of euthanizing laboratory animals are chosen to induce rapid unconsciousness and death without pain or distress. The methods that are preferred are those published by councils of veterinarians. The animal can be made to inhale a gas, such as carbon monoxide and carbon dioxide, by being placed in a chamber, or by use of a face mask, with or without prior sedation or anesthesia. Sedatives or anesthetics such as barbiturates can be given intravenously, or inhalant anesthetics may be used. Amphibians and fish may be immersed in water containing an anesthetic such as tricaine. Physical methods are also used, with or without sedation or anesthesia depending on the method. Recommended methods include decapitation (beheading) for small rodents or rabbits. Cervical dislocation (breaking the neck or spine) may be used for birds, mice, and immature rats and rabbits. High-intensity microwave irradiation of the brain can preserve brain tissue and induce death in less than 1 second, but this is currently only used on rodents. Captive bolts may be used, typically on dogs, ruminants, horses, pigs and rabbits. It causes death by a concussion to the brain. Gunshot may be used, but only in cases where a penetrating captive bolt may not be used. Some physical methods are only acceptable after the animal is unconscious. Electrocution may be used for cattle, sheep, swine, foxes, and mink after the animals are unconscious, often by a prior electrical stun. Pithing (inserting a tool into the base of the brain) is usable on animals already unconscious. Slow or rapid freezing, or inducing air embolism are acceptable only with prior anesthesia to induce unconsciousness.
Basic or pure research investigates how organisms behave, develop, and function. Those opposed to animal testing object that pure research may have little or no practical purpose, but researchers argue that it forms the necessary basis for the development of applied research, rendering the distinction between pure and applied research—research that has a specific practical aim—unclear. Pure research uses larger numbers and a greater variety of animals than applied research. Fruit flies, nematode worms, mice and rats together account for the vast majority, though small numbers of other species are used, ranging from sea slugs through to armadillos. Examples of the types of animals and experiments used in basic research include:
- Studies on embryogenesis and developmental biology. Mutants are created by adding transposons into their genomes, or specific genes are deleted by gene targeting. By studying the changes in development these changes produce, scientists aim to understand both how organisms normally develop, and what can go wrong in this process. These studies are particularly powerful since the basic controls of development, such as the homeobox genes, have similar functions in organisms as diverse as fruit flies and man.
- Experiments into behavior, to understand how organisms detect and interact with each other and their environment, in which fruit flies, worms, mice, and rats are all widely used. Studies of brain function, such as memory and social behavior, often use rats and birds. For some species, behavioral research is combined with enrichment strategies for animals in captivity because it allows them to engage in a wider range of activities.
- Breeding experiments to study evolution and genetics. Laboratory mice, flies, fish, and worms are inbred through many generations to create strains with defined characteristics. These provide animals of a known genetic background, an important tool for genetic analyses. Larger mammals are rarely bred specifically for such studies due to their slow rate of reproduction, though some scientists take advantage of inbred domesticated animals, such as dog or cattle breeds, for comparative purposes. Scientists studying how animals evolve use many animal species to see how variations in where and how an organism lives (their niche) produce adaptations in their physiology and morphology. As an example, sticklebacks are now being used to study how many and which types of mutations are selected to produce adaptations in animals' morphology during the evolution of new species.
Applied research aims to solve specific and practical problems. These may involve the use of animal models of diseases or conditions, which are often discovered or generated by pure research programmes. In turn, such applied studies may be an early stage in the drug discovery process. Examples include:
- Genetic modification of animals to study disease. Transgenic animals have specific genes inserted, modified or removed, to mimic specific conditions such as single gene disorders, such as Huntington's disease. Other models mimic complex, multifactorial diseases with genetic components, such as diabetes, or even transgenic mice that carry the same mutations that occur during the development of cancer. These models allow investigations on how and why the disease develops, as well as providing ways to develop and test new treatments. The vast majority of these transgenic models of human disease are lines of mice, the mammalian species in which genetic modification is most efficient. Smaller numbers of other animals are also used, including rats, pigs, sheep, fish, birds, and amphibians.
- Studies on models of naturally occurring disease and condition. Certain domestic and wild animals have a natural propensity or predisposition for certain conditions that are also found in humans. Cats are used as a model to develop immunodeficiency virus vaccines and to study leukemia because their natural predisposition to FIV and Feline leukemia virus. Certain breeds of dog suffer from narcolepsy making them the major model used to study the human condition. Armadillos and humans are among only a few animal species that naturally suffer from leprosy; as the bacteria responsible for this disease cannot yet be grown in culture, armadillos are the primary source of bacilli used in leprosy vaccines.
- Studies on induced animal models of human diseases. Here, an animal is treated so that it develops pathology and symptoms that resemble a human disease. Examples include restricting blood flow to the brain to induce stroke, or giving neurotoxins that cause damage similar to that seen in Parkinson's disease. Much animal research into potential treatments for humans is wasted because it is poorly conducted and not evaluated through systematic reviews. For example, although such models are now widely used to study Parkinson's disease, the British anti-vivisection interest group BUAV argues that these models only superficially resemble the disease symptoms, without the same time course or cellular pathology. In contrast, scientists assessing the usefulness of animal models of Parkinson's disease, as well as the medical research charity The Parkinson's Appeal, state that these models were invaluable and that they led to improved surgical treatments such as pallidotomy, new drug treatments such as levodopa, and later deep brain stimulation.
- Animal testing has also included the use of placebo testing. In these cases animals are treated with a substance that produces no pharmacological effect, but is administered in order to determine any biological alterations due to the experience of a substance being administered, and the results are compared with those obtained with an active compound.
Xenotransplantation research involves transplanting tissues or organs from one species to another, as a way to overcome the shortage of human organs for use in organ transplants. Current research involves using primates as the recipients of organs from pigs that have been genetically modified to reduce the primates' immune response against the pig tissue. Although transplant rejection remains a problem, recent clinical trials that involved implanting pig insulin-secreting cells into diabetics did reduce these people's need for insulin.
Documents released to the news media by the animal rights organization Uncaged Campaigns showed that, between 1994 and 2000, wild baboons imported to the UK from Africa by Imutran Ltd, a subsidiary of Novartis Pharma AG, in conjunction with Cambridge University and Huntingdon Life Sciences, to be used in experiments that involved grafting pig tissues, suffered serious and sometimes fatal injuries. A scandal occurred when it was revealed that the company had communicated with the British government in an attempt to avoid regulation.
Toxicology testing, also known as safety testing, is conducted by pharmaceutical companies testing drugs, or by contract animal testing facilities, such as Huntingdon Life Sciences, on behalf of a wide variety of customers. According to 2005 EU figures, around one million animals are used every year in Europe in toxicology tests; which are about 10% of all procedures. According to Nature, 5,000 animals are used for each chemical being tested, with 12,000 needed to test pesticides. The tests are conducted without anesthesia, because interactions between drugs can affect how animals detoxify chemicals, and may interfere with the results.
Toxicology tests are used to examine finished products such as pesticides, medications, food additives, packing materials, and air freshener, or their chemical ingredients. Most tests involve testing ingredients rather than finished products, but according to BUAV, manufacturers believe these tests overestimate the toxic effects of substances; they therefore repeat the tests using their finished products to obtain a less toxic label.
The substances are applied to the skin or dripped into the eyes; injected intravenously, intramuscularly, or subcutaneously; inhaled either by placing a mask over the animals and restraining them, or by placing them in an inhalation chamber; or administered orally, through a tube into the stomach, or simply in the animal's food. Doses may be given once, repeated regularly for many months, or for the lifespan of the animal.
There are several different types of acute toxicity tests. The LD50 ("Lethal Dose 50%") test is used to evaluate the toxicity of a substance by determining the dose required to kill 50% of the test animal population. This test was removed from OECD international guidelines in 2002, replaced by methods such as the fixed dose procedure, which use fewer animals and cause less suffering. Abbott writes that, as of 2005, "the LD50 acute toxicity test ... still accounts for one-third of all animal [toxicity] tests worldwide".
Irritancy can be measured using the Draize test, where a test substance is applied to an animal's eyes or skin, usually an albino rabbit. For Draize eye testing, the test involves observing the effects of the substance at intervals and grading any damage or irritation, but the test should be halted and the animal killed if it shows "continuing signs of severe pain or distress". The Humane Society of the United States writes that the procedure can cause redness, ulceration, hemorrhaging, cloudiness, or even blindness. This test has also been criticized by scientists for being cruel and inaccurate, subjective, over-sensitive, and failing to reflect human exposures in the real world. Although no accepted in vitro alternatives exist, a modified form of the Draize test called the low volume eye test may reduce suffering and provide more realistic results and this was adopted as the new standard in September 2009. However, the Draize test will still be used for substances that are not severe irritants.
The most stringent tests are reserved for drugs and foodstuffs. For these, a number of tests are performed, lasting less than a month (acute), one to three months (subchronic), and more than three months (chronic) to test general toxicity (damage to organs), eye and skin irritancy, mutagenicity, carcinogenicity, teratogenicity, and reproductive problems. The cost of the full complement of tests is several million dollars per substance and it may take three or four years to complete.
These toxicity tests provide, in the words of a 2006 United States National Academy of Sciences report, "critical information for assessing hazard and risk potential". Animal tests may overestimate risk, with false positive results being a particular problem, but false positives appear not to be prohibitively common. Variability in results arises from using the effects of high doses of chemicals in small numbers of laboratory animals to try to predict the effects of low doses in large numbers of humans. Although relationships do exist, opinion is divided on how to use data on one species to predict the exact level of risk in another.
Scientists face growing pressure to move away from using traditional animal toxicity tests to determine whether manufactured chemicals are safe. Among variety of approaches to toxicity evaluation the ones which have attracted increasing interests are in vitro cell-based sensing methods applying fluorescence.
Cosmetics testing on animals is particularly controversial. Such tests, which are still conducted in the U.S., involve general toxicity, eye and skin irritancy, phototoxicity (toxicity triggered by ultraviolet light) and mutagenicity.
Cosmetics testing on animals is banned in India, the European Union, Israel and Norway while legislation in the U.S. and Brazil is currently considering similar bans. In 2002, after 13 years of discussion, the European Union agreed to phase in a near-total ban on the sale of animal-tested cosmetics by 2009, and to ban all cosmetics-related animal testing. France, which is home to the world's largest cosmetics company, L'Oreal, has protested the proposed ban by lodging a case at the European Court of Justice in Luxembourg, asking that the ban be quashed. The ban is also opposed by the European Federation for Cosmetics Ingredients, which represents 70 companies in Switzerland, Belgium, France, Germany, and Italy. In October 2014, India passed stricter laws that also ban the importation of any cosmetic products that are tested on animals.
Before the early 20th century, laws regulating drugs were lax. Currently, all new pharmaceuticals undergo rigorous animal testing before being licensed for human use. Tests on pharmaceutical products involve:
- metabolic tests, investigating pharmacokinetics—how drugs are absorbed, metabolized and excreted by the body when introduced orally, intravenously, intraperitoneally, intramuscularly, or transdermally.
- toxicology tests, which gauge acute, sub-acute, and chronic toxicity. Acute toxicity is studied by using a rising dose until signs of toxicity become apparent. Current European legislation demands that "acute toxicity tests must be carried out in two or more mammalian species" covering "at least two different routes of administration". Sub-acute toxicity is where the drug is given to the animals for four to six weeks in doses below the level at which it causes rapid poisoning, in order to discover if any toxic drug metabolites build up over time. Testing for chronic toxicity can last up to two years and, in the European Union, is required to involve two species of mammals, one of which must be non-rodent.
- efficacy studies, which test whether experimental drugs work by inducing the appropriate illness in animals. The drug is then administered in a double-blind controlled trial, which allows researchers to determine the effect of the drug and the dose-response curve.
- Specific tests on reproductive function, embryonic toxicity, or carcinogenic potential can all be required by law, depending on the result of other studies and the type of drug being tested.
It is estimated that 20 million animals are used annually for educational purposes in the United States including, classroom observational exercises, dissections and live-animal surgeries. Frogs, fetal pigs, perch, cats, earthworms, grasshoppers, crayfish and starfish are commonly used in classroom dissections. Alternatives to the use of animals in classroom dissections are widely used, with many U.S. States and school districts mandating students be offered the choice to not dissect. Citing the wide availability of alternatives and the decimation of local frog species, India banned dissections in 2014.
The Sonoran Arthropod Institute hosts an annual Invertebrates in Education and Conservation Conference to discuss the use of invertebrates in education. There also are efforts in many countries to find alternatives to using animals in education. The NORINA database, maintained by Norecopa, lists products that may be used as alternatives or supplements to animal use in education, and in the training of personnel who work with animals. These include alternatives to dissection in schools. InterNICHE has a similar database and a loans system.
In November 2013, the U.S.-based company Backyard Brains released for sale to the public what they call the "Roboroach", an "electronic backpack" that can be attached to cockroaches. The operator is required to amputate a cockroach's antennae, use sandpaper to wear down the shell, insert a wire into the thorax, and then glue the electrodes and circuit board onto the insect's back. A mobile phone app can then be used to control it via Bluetooth. It has been suggested that the use of such a device may be a teaching aid that can promote interest in science. The makers of the "Roboroach" have been funded by the National Institute of Mental Health and state that the device is intended to encourage children to become interested in neuroscience.
Animals are used by the military to develop weapons, vaccines, battlefield surgical techniques, and defensive clothing. For example, in 2008 the United States Defense Advanced Research Projects Agency used live pigs to study the effects of improvised explosive device explosions on internal organs, especially the brain.
In the US military, goats are commonly used to train combat medics. (Goats have become the main animal species used for this purpose after the Pentagon phased out using dogs for medical training in the 1980s.) While modern mannequins used in medical training are quite efficient in simulating the behavior of a human body, some trainees feel that "the goat exercise provide[s] a sense of urgency that only real life trauma can provide". Nevertheless, in 2014, the U.S. Coast Guard announced that it would reduce the number of animals it uses in its training exercises by half after PETA released video showing Guard members cutting off the limbs of unconscious goats with tree trimmers and inflicting other injuries with a shotgun, pistol, ax and a scalpel. That same year, citing the availability of human simulators and other alternatives, the Department of Defense announced it would begin reducing the number of animals it uses in various training programs. In 2013, several Navy medical centers stopped using ferrets in intubation exercises after complaints from PETA.
Besides the United States, six out of 28 NATO countries, including Poland and Denmark, use live animals for combat medic training.
The moral and ethical questions raised by performing experiments on animals are subject to debate, and viewpoints have shifted significantly over the 20th century. There remain disagreements about which procedures are useful for which purposes, as well as disagreements over which ethical principles apply to which species.
A 2015 Gallup poll found that 67% of Americans were "very concerned" or "somewhat concerned" about animals used in research. A Pew poll taken the same year found 50% of American adults opposed the use of animals in research.
Still, a wide range of viewpoints exist. The view that animals have moral rights (animal rights) is a philosophical position proposed by Tom Regan, among others, who argues that animals are beings with beliefs and desires, and as such are the "subjects of a life" with moral value and therefore moral rights. Regan still sees ethical differences between killing human and non-human animals, and argues that to save the former it is permissible to kill the latter. Likewise, a "moral dilemma" view suggests that avoiding potential benefit to humans is unacceptable on similar grounds, and holds the issue to be a dilemma in balancing such harm to humans to the harm done to animals in research. In contrast, an abolitionist view in animal rights holds that there is no moral justification for any harmful research on animals that is not to the benefit of the individual animal. Bernard Rollin argues that benefits to human beings cannot outweigh animal suffering, and that human beings have no moral right to use an animal in ways that do not benefit that individual. Donald Watson has stated that vivisection and animal experimentation "is probably the cruelest of all Man's attack on the rest of Creation." Another prominent position is that of philosopher Peter Singer, who argues that there are no grounds to include a being's species in considerations of whether their suffering is important in utilitarian moral considerations. Malcolm Macleod and collaborators argue that most controlled animal studies do not employ randomization, allocation concealment, and blinding outcome assessment, and that failure to employ these features exaggerates the apparent benefit of drugs tested in animals, leading to a failure to translate much animal research for human benefit.
Governments such as the Netherlands and New Zealand have responded to the public's concerns by outlawing invasive experiments on certain classes of non-human primates, particularly the great apes. In 2015, captive chimpanzees in the U.S. were added to the Endangered Species Act adding new road blocks to those wishing to experiment on them. Similarly, citing ethical considerations and the availability of alternative research methods, the U.S. NIH announced in 2013 that it would dramatically reduce and eventually phase out experiments on chimpanzees.
The British government has required that the cost to animals in an experiment be weighed against the gain in knowledge. Some medical schools and agencies in China, Japan, and South Korea have built cenotaphs for killed animals. In Japan there are also annual memorial services (Ireisai 慰霊祭) for animals sacrificed at medical school.
Various specific cases of animal testing have drawn attention, including both instances of beneficial scientific research, and instances of alleged ethical violations by those performing the tests. The fundamental properties of muscle physiology were determined with work done using frog muscles (including the force generating mechanism of all muscle, the length-tension relationship, and the force-velocity curve), and frogs are still the preferred model organism due to the long survival of muscles in vitro and the possibility of isolating intact single-fiber preparations (not possible in other organisms). Modern physical therapy and the understanding and treatment of muscular disorders is based on this work and subsequent work in mice (often engineered to express disease states such as muscular dystrophy). In February 1997 a team at the Roslin Institute in Scotland announced the birth of Dolly the sheep, the first mammal to be cloned from an adult somatic cell.
Concerns have been raised over the mistreatment of primates undergoing testing. In 1985 the case of Britches, a macaque monkey at the University of California, Riverside, gained public attention. He had his eyelids sewn shut and a sonar sensor on his head as part of an experiment to test sensory substitution devices for blind people. The laboratory was raided by Animal Liberation Front in 1985, removing Britches and 466 other animals. The National Institutes of Health conducted an eight-month investigation and concluded, however, that no corrective action was necessary. During the 2000s other cases have made headlines, including experiments at the University of Cambridge and Columbia University in 2002. In 2004 and 2005, undercover footage of staff of Covance's, a contract research organization that provides animal testing services, Virginia lab was shot by People for the Ethical Treatment of Animals (PETA). Following release of the footage, the U.S. Department of Agriculture fined Covance $8,720 for 16 citations, three of which involved lab monkeys; the other citations involved administrative issues and equipment.
Threats to researchersEdit
In 2006, a primate researcher at the University of California, Los Angeles (UCLA) shut down the experiments in his lab after threats from animal rights activists. The researcher had received a grant to use 30 macaque monkeys for vision experiments; each monkey was anesthetized for a single physiological experiment lasting up to 120 hours, and then euthanized. The researcher's name, phone number, and address were posted on the website of the Primate Freedom Project. Demonstrations were held in front of his home. A Molotov cocktail was placed on the porch of what was believed to be the home of another UCLA primate researcher; instead, it was accidentally left on the porch of an elderly woman unrelated to the university. The Animal Liberation Front claimed responsibility for the attack. As a result of the campaign, the researcher sent an email to the Primate Freedom Project stating "you win", and "please don't bother my family anymore". In another incident at UCLA in June 2007, the Animal Liberation Brigade placed a bomb under the car of a UCLA children's ophthalmologist who experiments on cats and rhesus monkeys; the bomb had a faulty fuse and did not detonate.
In 1997, PETA filmed staff from Huntingdon Life Sciences, showing dogs being mistreated. The employees responsible were dismissed, with two given community service orders and ordered to pay £250 costs, the first lab technicians to have been prosecuted for animal cruelty in the UK. The Stop Huntingdon Animal Cruelty campaign used tactics ranging from non-violent protest to the alleged firebombing of houses owned by executives associated with HLS's clients and investors. The Southern Poverty Law Center, which monitors US domestic extremism, has described SHAC's modus operandi as "frankly terroristic tactics similar to those of anti-abortion extremists," and in 2005 an official with the FBI's counter-terrorism division referred to SHAC's activities in the United States as domestic terrorist threats. 13 members of SHAC were jailed for between 15 months and eleven years on charges of conspiracy to blackmail or harm HLS and its suppliers.
These attacks—as well as similar incidents that caused the Southern Poverty Law Center to declare in 2002 that the animal rights movement had "clearly taken a turn toward the more extreme"—prompted the US government to pass the Animal Enterprise Terrorism Act and the UK government to add the offense of "Intimidation of persons connected with animal research organisation" to the Serious Organised Crime and Police Act 2005. Such legislation and the arrest and imprisonment of activists may have decreased the incidence of attacks.
Systematic reviews have pointed out that animal testing often fails to accurately mirror outcomes in humans. For instance, a 2013 review noted that some 100 vaccines have been shown to prevent HIV in animals, yet none of them have worked on humans. Effects seen in animals may not be replicated in humans, and vice versa. Many corticosteroids cause birth defects in animals, but not in humans. Conversely, thalidomide causes serious birth defects in humans, but not in animals. A 2004 paper concluded that much animal research is wasted because systemic reviews are not used, and due to poor methodology. A 2006 review found multiple studies where there were promising results for new drugs in animals, but human clinical studies did not show the same results. The researchers suggested that this might be due to researcher bias, or simply because animal models do not accurately reflect human biology. Lack of meta-reviews may be partially to blame. Poor methodology is an issue in many studies. A 2009 review noted that many animal experiments did not use blinded experiments, a key element of many scientific studies in which researchers are not told about the part of the study they are working on to reduce bias.
Alternatives to animal testingEdit
Most scientists and governments state that animal testing should cause as little suffering to animals as possible, and that animal tests should only be performed where necessary. The "Three Rs" are guiding principles for the use of animals in research in most countries. Whilst replacement of animals, i.e. alternatives to animal testing, is one of the principles, their scope is much broader. Although such principles have been welcomed as a step forwards by some animal welfare groups, they have also been criticized as both outdated by current research, and of little practical effect in improving animal welfare.
The scientists and engineers at Harvard's Wyss Institute have created "organs-on-a-chip", including the "lung-on-a-chip" and "gut-on-a-chip". Researchers at cellasys in Germany developed a "skin-on-a-chip". These tiny devices contain human cells in a 3-dimensional system that mimics human organs. The chips can be used instead of animals in in vitro disease research, drug testing, and toxicity testing. Researchers have also begun using 3-D bioprinters to create human tissues for in vitro testing.
Another non-animal research method is in silico or computer simulation and mathematical modeling which seeks to investigate and ultimately predict toxicity and drug affects in humans without using animals. This is done by investigating test compounds on a molecular level using recent advances in technological capabilities with the ultimate goal of creating treatments unique to each patient.
Microdosing is another alternative to the use of animals in experimentation. Microdosing is a process whereby volunteers are administered a small dose of a test compound allowing researchers to investigate its pharmacological affects without harming the volunteers. Microdosing can replace the use of animals in pre-clinical drug screening and can reduce the number of animals used in safety and toxicity testing.
Additional alternative methods include positron emission tomography (PET), which allows scanning of the human brain in vivo, and comparative epidemiological studies of disease risk factors among human populations.
Official bodies such as the European Centre for the Validation of Alternative Test Methods of the European Commission, the Interagency Coordinating Committee for the Validation of Alternative Methods in the US, ZEBET in Germany, and the Japanese Center for the Validation of Alternative Methods (among others) also promote and disseminate the 3Rs. These bodies are mainly driven by responding to regulatory requirements, such as supporting the cosmetics testing ban in the EU by validating alternative methods.
The European Partnership for Alternative Approaches to Animal Testing serves as a liaison between the European Commission and industries. The European Consensus Platform for Alternatives coordinates efforts amongst EU member states.
- ""Introduction", Select Committee on Animals in Scientific Procedures Report". UK Parliament. Retrieved 13 July 2012.
- Meredith Cohn (26 August 2010). "Alternatives to Animal Testing Gaining Ground," The Baltimore Sun.
- Carbone, Larry. (2004). What Animals Want: Expertise and Advocacy in Laboratory Animal Welfare Policy.
- "EU statistics show decline in animal research numbers". Speaking of Research. 2013. Retrieved 24 January 2016.
- Carbone, p. 22.
- "Use of Laboratory Animals in Biomedical and Behavioral Research", Institute for Laboratory Animal Research, The National Academies Press, 1988 ISBN 0-309-07878-4.
- Cooper, Sylvia (1 August 1999). "Pets crowd animal shelter", The Augusta Chronicle.
- Gillham, Christina (17 February 2006). "Bought to be sold", Newsweek.
- The use of non-human animals in research: a guide for scientists The Royal Society, 2004, p. 1
- "Science, Medicine, and Animals", Institute for Laboratory Animal Research, Published by the National Research Council of the National Academies 2004, p. 2
- Croce, Pietro (1999). Vivisection or Science? An Investigation into Testing Drugs and Safeguarding Health. Zed Books, ISBN 1-85649-732-1.
- "About". Peta.org. Retrieved 6 April 2015.
- "UK Legislation: A Criticism" (PDF). Web.archive.org. Archived from the original (PDF) on 25 June 2008. Retrieved 6 April 2015.
- "FAQs: Vivisection" (PDF). British Union for the Abolition of Vivisection. Archived from the original (PDF) on 13 May 2015. Retrieved 6 April 2015.
- "Biomedical Research : The Humane Society of the United States". Humanesociety.org. Retrieved 6 April 2015.
- "Animal Testing and Animal Experimentation Issues | Physicians Committee". Pcrm.org. Archived from the original on 23 July 2011. Retrieved 6 April 2015.
- "Vivisection" Archived 1 January 2008 at the Wayback Machine, Encyclopædia Britannica, 2007.
- "Vivisection FAQ" (PDF). British Union for the Abolition of Vivisection. Archived from the original (PDF) on 13 May 2015.
- Definitions in:
- Paixão RL, Schramm FR (1999). "Ethics and animal experimentation: what is debated?". Cadernos de Saúde Pública. 15 (Suppl 1): 99–110. doi:10.1590/s0102-311x1999000500011. PMID 10089552.
- Yarri, Donna (2005). The Ethics of Animal Experimentation, Oxford University Press U.S., ISBN 0-19-518179-4.
- Cohen and Loew 1984.
- "History of nonhuman animal research". Laboratory Primate Advocacy Group. Archived from the original on 13 October 2006.
- Abdel-Halim RE (2005). "Contributions of Ibn Zuhr (Avenzoar) to the progress of surgery: a study and translations from his book Al-Taisir". Saudi Medical Journal. 26 (9): 1333–39. PMID 16155644.
- Abdel-Halim RE (2006). "Contributions of Muhadhdhab Al-Deen Al-Baghdadi to the progress of medicine and urology. A study and translations from his book Al-Mukhtar". Saudi Medical Journal. 27 (11): 1631–41. PMID 17106533.
- Costello, John (9 June 2011). "The great zoo's who". Irish Independent.
- Mock M, Fouet A (2001). "Anthrax". Annu. Rev. Microbiol. 55: 647–71. doi:10.1146/annurev.micro.55.1.647. PMID 11544370.
- Windholz G (1987). "Pavlov as a psychologist. A reappraisal". Pavlovian J. Biol. Sci. 22 (3): 103–12. PMID 3309839.
- Maczulak, Anne (2011), "bioweapon", Encyclopedia of Microbiology, Facts On File, pp. 127–35, ISBN 978-0-8160-7364-1
- Gorden P (1997). "Non-insulin dependent diabetes – the past, present and future". Ann. Acad. Med. Singap. 26 (3): 326–30. PMID 9285027.
- Walgate R (1981). "Armadillos fight leprosy". Nature. 291 (5816): 527. Bibcode:1981Natur.291..527W. doi:10.1038/291527a0. PMID 7242665.
- Scollard DM, Adams LB, Gillis TP, Krahenbuhl JL, Truman RW, Williams DL (2006). "The Continuing Challenges of Leprosy". Clin. Microbiol. Rev. 19 (2): 338–81. doi:10.1128/CMR.19.2.338-381.2006. PMC 1471987. PMID 16614253.
- Jaenisch R, Mintz B (1974). "Simian Virus 40 DNA Sequences in DNA of Healthy Adult Mice Derived from Preimplantation Blastocysts Injected with Viral DNA". Proceedings of the National Academy of Sciences of the United States of America. 71 (4): 1250–54. Bibcode:1974PNAS...71.1250J. doi:10.1073/pnas.71.4.1250. PMC 388203. PMID 4364530.
- Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997). "Viable offspring derived from fetal and adult mammalian cells". Nature. 385 (6619): 810–13. Bibcode:1997Natur.385..810W. doi:10.1038/385810a0. PMID 9039911. S2CID 4260518.
- "History of animal research". www.understandinganimalresearch.org.uk. Retrieved 8 April 2016.
- "Taste of Raspberries, Taste of Death. The 1937 Elixir Sulfanilamide Incident". FDA Consumer magazine. June 1981.
- Burkholz, Herbert (1 September 1997). "Giving Thalidomide a Second Chance". FDA Consumer. US Food and Drug Administration.
- Croce, Pietro. Vivisection or Science? An Investigation into Testing Drugs and Safeguarding Health. Zed Books, 1999, ISBN 1-85649-732-1 p. 11.
- Bernard, Claude An Introduction to the Study of Experimental Medicine, 1865. First English translation by Henry Copley Greene, published by Macmillan & Co., Ltd., 1927; reprinted in 1949, p. 125.
- Ryder, Richard D. (2000). Animal Revolution: Changing Attitudes Towards Speciesism. Berg Publishers, p. 54 ISBN 1-85973-330-1.
- "Animal Experimentation: A Student Guide to Balancing the Issues", Australian and New Zealand Council for the Care of Animals in Research and Teaching (ANZCCART), accessed 12 December 2007, cites original reference in Maehle, A-H. and Tr6hler, U. Animal experimentation from antiquity to the end of the eighteenth century: attitudes and arguments. In N. A. Rupke (ed.) Vivisection in Historical Perspective. Croom Helm, London, 1987, p. 22.
- Rudacille, Deborah (2000). The Scalpel and the Butterfly: The Conflict, University of California Press, p. 19 ISBN 0-520-23154-6.
- "In sickness and in health: vivisection's undoing", The Daily Telegraph, November 2003
- LaFollette, H., Shanks, N., Animal Experimentation: the Legacy of Claude Bernard Archived 10 January 2020 at the Wayback Machine, International Studies in the Philosophy of Science (1994) pp. 195–210.
- Nicoll CS (1991). "A Physiologist's Views on the Animal Rights/Liberation Movement". The Physiologist. 34 (6): 303, 306–08, 315. PMID 1775539.
- Mason, Peter. The Brown Dog Affair. Two Sevens Publishing, 1997.
- "The Life and Letters of Charles Darwin, Volume II". Fullbooks.com.
- Bowlby, John (1991). Charles Darwin: A New Life, W. W. Norton & Company, p. 420 ISBN 0-393-30930-4.
- Ilman, John (2008). Animal Research in Medicine: 100 years of politics, protest and progress. The Story of the Research Defence Society. Research Defence Society. p. 16. ISBN 978-0-9560008-0-4.
- Publications of the Research Defence Society: March 1908–1909; Selected by the committee. London: Macmillan. 1909. p. xiv.
- Buettinger, Craig (1 January 1993) Antivivisection and the charge of zoophil-psychosis in the early twentieth century. The Historian.
- Carbone, pp. 68–69.
- Office of Laboratory Animal Welfare. Public Health Service Policy on Humane Care and Use of Laboratory Animals. nih.gov
- Title 9 – Animals and Animal Products. Code of Federal Regulations. Vol. 1 (1 January 2008).
- "Animal Testing and the Law - Animal Legal Defense Fund". Animal Legal Defense Fund. Archived from the original on 23 August 2017. Retrieved 14 June 2017.
- Harden, Gil. "USDA Inspector General Audit Report of APHIS Animal Care Program Inspection and Enforcement Activities" (PDF). United States Department of Agriculture Office of Inspector General (Report No. 33601–0001–41). Retrieved 7 July 2015.
- Young, Robert (September 2005). "Audit Report: APHIS Animal Care Program Inspection and Enforcement Activities" (PDF). USDA Office of Inspector General Western Region (Report No. 33002–3–SF). Retrieved 7 July 2015.
- Hansen, L; Goodman, J; Chandna, A (2012). "Analysis of animal research ethics committee membership at American institutions". Animals. 2 (1): 68–75. doi:10.3390/ani2010068. PMC 4494267. PMID 26486777.
- Carbone, p. 94.
- Plous S, Herzog H (2001). "Animal Research: Reliability of Protocol Reviews for Animal Research". Science. 293 (5530): 608–09. doi:10.1126/science.1061621. PMID 11474086. S2CID 33314019.
- Nandi, Jayashree (27 April 2012). "Scientists take on activists, want ban on live testing on animals lifted". The Times of India. Archived from the original on 27 October 2012. Retrieved 13 July 2012.
- Taylor, Katy; Gordon, Nicky; Langley, Gill; Higgins, Wendy (2008). "Estimates for worldwide laboratory animal use in 2005". ATLA. 36 (3): 327–42. doi:10.1177/026119290803600310. PMID 18662096. S2CID 196613886.
- Hunter, Robert G. (1 January 2014). "Alternatives to animal testing drive market". Gen. Eng. Biotechnol. News. 34 (1). p. 11.
While growth has leveled off and there have been significant reductions in some countries, the number of animals used in research globally still totals almost 100 million a year.
- "The Ethics of research involving animals" (PDF). Nuffield Council on Bioethics. Archived from the original (PDF) on 25 June 2008.
- "USDA publishes 2016 animal research statistics – 7% rise in animal use". Speaking of Research. 19 June 2017. Retrieved 10 December 2017.
- "USDA Statistics for Animals Used in Research in the US". Speaking of Research. 20 March 2008.
- Goodman, J.; Chandna, A.; Roe, K. (2015). "Trends in animal use at US research facilities". Journal of Medical Ethics. 41 (7): 567–69. doi:10.1136/medethics-2014-102404. PMID 25717142. S2CID 46187262. Retrieved 7 July 2015.
- Rowan, A., Loew, F., and Weer, J. (1995) "The Animal Research Controversy. Protest, Process and Public Policy: An Analysis of Strategic Issues." Tufts University, North Grafton. cited in Carbone 2004, p. 26.
- Alternatives to Animal Use in Research, Testing and Education, U.S. Congress Office of Technology Assessment, Washington, D.C.:Government Printing Office, 1986, p. 64. In 1966, the Laboratory Animal Breeders Association estimated in testimony before Congress that the number of mice, rats, guinea pigs, hamsters, and rabbits used in 1965 was around 60 million. (Hearings before the Subcommittee on Livestock and Feed Grains, Committee on Agriculture, U.S. House of Representatives, 1966, p. 63.)
- "Animal research numbers in 2017". Understanding Animal Research. 2017.
- "Home Office Statistics for Animals Used in Research in the UK". Speaking of Research. 23 October 2012.
- Russell, W. M. S. (William Moy Stratton); Health, JH Bloomberg School of Public (1992). The principles of humane experimental technique (Special ed.). South Mimms, Potters Bar, Herts, England: Universities Federation for Animal Welfare. ISBN 0900767782. OCLC 27347928.
- Badyal D., Desai C. (2014). "Animal use in pharmacology education and research: The changing scenario". Indian Journal of Pharmacology. 46 (3): 257–65. doi:10.4103/0253-7613.132153. PMC 4071700. PMID 24987170.CS1 maint: uses authors parameter (link)
- Liguori, G.; et al. (2017). "Ethical Issues in the Use of Animal Models for Tissue Engineering: Reflections on Legal Aspects, Moral Theory, 3Rs Strategies, and Harm-Benefit Analysis" (PDF). Tissue Engineering Part C: Methods. 23 (12): 850–62. doi:10.1089/ten.TEC.2017.0189. PMID 28756735.
- Canadian Council on Animal Care (December 2010). "2009 CCAC Survey of Animal Use" (PDF). Archived from the original (PDF) on 7 June 2015. Retrieved 7 July 2015. Cite journal requires
- Merkes, M.; Buttrose, R. "New code, same suffering: animals in the lab". ABC. The Drum. Retrieved 7 July 2015.
- Even, Dan (29 May 2013). "Number of animal experiments up for first time since 2008". Haaretz. Retrieved 7 July 2015.
- "Rise in animal research in South Korea in 2017". 20 April 2018. Retrieved 23 July 2017. Cite journal requires
- "Number of laboratory animals in Germany". Max-Planck-Gesellschaft. Max-Planck-Gesellschaft. Retrieved 7 July 2015.
- Kong, Q.; Qin, C. (2009). "Analysis of current laboratory animal science policies and administration in China". ILAR. 51 (1): e1–e11. doi:10.1093/ilar.51.1.e1. PMID 20075493.
- Antoshechkin I, Sternberg PW (2007). "The versatile worm: genetic and genomic resources for Caenorhabditis elegans research". Nature Reviews Genetics. 8 (7): 518–32. doi:10.1038/nrg2105. PMID 17549065. S2CID 12923468.
- Matthews KA, Kaufman TC, Gelbart WM (2005). "Research resources for Drosophila: the expanding universe". Nature Reviews Genetics. 6 (3): 179–93. doi:10.1038/nrg1554. PMID 15738962. S2CID 31002250.
- Schulenburg H, Kurz CL, Ewbank JJ (2004). "Evolution of the innate immune system: the worm perspective". Immunological Reviews. 198: 36–58. doi:10.1111/j.0105-2896.2004.0125.x. PMID 15199953.
- Leclerc V, Reichhart JM (2004). "The immune response of Drosophila melanogaster". Immunological Reviews. 198: 59–71. doi:10.1111/j.0105-2896.2004.0130.x. PMID 15199954.
- Mylonakis E, Aballay A (2005). "Worms and flies as genetically tractable animal models to study host-pathogen interactions". Infection and Immunity. 73 (7): 3833–41. doi:10.1128/IAI.73.7.3833-3841.2005. PMC 1168613. PMID 15972468.
- Kavanagh K, Reeves EP (2004). "Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens". FEMS Microbiology Reviews. 28 (1): 101–12. doi:10.1016/j.femsre.2003.09.002. PMID 14975532.
- Antunes LC, Imperi F, Carattoli A, Visca P (2011). Adler B (ed.). "Deciphering the Multifactorial Nature of Acinetobacter baumannii Pathogenicity". PLOS One. 6 (8): e22674. Bibcode:2011PLoSO...622674A. doi:10.1371/journal.pone.0022674. PMC 3148234. PMID 21829642.
- Aperis G, Fuchs BB, Anderson CA, Warner JE, Calderwood SB, Mylonakis E (2007). "Galleria mellonella as a model host to study infection by the Francisella tularensis live vaccine strain". Microbes and Infection / Institut Pasteur. 9 (6): 729–34. doi:10.1016/j.micinf.2007.02.016. PMC 1974785. PMID 17400503.
- Waterfield NR, Sanchez-Contreras M, Eleftherianos I, Dowling A, Yang G, Wilkinson P, Parkhill J, Thomson N, Reynolds SE, Bode HB, Dorus S, Ffrench-Constant RH (2008). "Rapid Virulence Annotation (RVA): Identification of virulence factors using a bacterial genome library and multiple invertebrate hosts". Proceedings of the National Academy of Sciences of the United States of America. 105 (41): 15967–72. Bibcode:2008PNAS..10515967W. doi:10.1073/pnas.0711114105. PMC 2572985. PMID 18838673.
- Trull, F. L. (1999). "More Regulation of Rodents". Science. 284 (5419): 1463. Bibcode:1999Sci...284.1463T. doi:10.1126/science.284.5419.1463. PMID 10383321. S2CID 10122407.
- Rosenthal N, Brown S (2007). "The mouse ascending: perspectives for human-disease models". Nature Cell Biology. 9 (9): 993–99. doi:10.1038/ncb437. PMID 17762889. S2CID 4472227.
- Mukerjee, M (August 2004). "Speaking for the Animals". Scientific American. 291 (2): 96–97. Bibcode:2004SciAm.291b..96M. doi:10.1038/scientificamerican0804-96.
- Aitman TJ, Critser JK, Cuppen E, Dominiczak A, Fernandez-Suarez XM, Flint J, Gauguier D, Geurts AM, Gould M, Harris PC, Holmdahl R, Hubner N, Izsvák Z, Jacob HJ, Kuramoto T, Kwitek AE, Marrone A, Mashimo T, Moreno C, Mullins J, Mullins L, Olsson T, Pravenec M, Riley L, Saar K, Serikawa T, Shull JD, Szpirer C, Twigger SN, Voigt B, Worley K (2008). "Progress and prospects in rat genetics: a community view". Nature Genetics. 40 (5): 516–22. doi:10.1038/ng.147. PMID 18443588. S2CID 22522876.
- "Statistics of Scientific Procedures on Living Animals, Great Britain" (PDF). UK Home Office. 2017. Retrieved 23 July 2018.
- "Statistics of Scientific Procedures on Living Animals, Great Britain" (PDF). British government. 2004. Retrieved 13 July 2012.
- Statistics of Scientific Procedures on Living Animals, Great Britain, 1996 - UK Home Office, Table 13
- "Annual Report Animals" (PDF). Aphis.usda.gov. Retrieved 6 August 2017.
- Dog profile, The Humane Society of the United States
- Smith, D; Broadhead, C; Descotes, G; Fosse, R; Hack, R; Krauser, K; Pfister, R; Phillips, B; Rabemampianina, Y; Sanders, J; Sparrow, S; Stephan-Gueldner, M; Jacobsen, SD (2002). "Preclinical Safety Evaluation Using Nonrodent Species: An Industry/ Welfare Project to Minimize Dog Use". ILAR. 43 Suppl: S39-42. doi:10.1093/ilar.43.Suppl_1.S39. PMID 12388850.
- Quianzon, Celeste C.; Cheikh, Issam (16 July 2012). "History of insulin". Journal of Community Hospital Internal Medicine Perspectives. 2 (2): 18701. doi:10.3402/jchimp.v2i2.18701. ISSN 2000-9666. PMC 3714061. PMID 23882369.
- "Germany sees 7% rise in animal research procedures in 2016". Speaking of Research. 6 February 2018.
- "France, Italy and the Netherlands publish their 2016 statistics". Speaking of Research. 20 March 2018.
- International Perspectives: The Future of Nonhuman Primate Resources, Proceedings of the Workshop Held 17–19 April, pp. 36–45, 46–48, 63–69, 197–200.
- "Seventh Report on the Statistics on the Number of Animals used for Experimental and other Scientific Purposes in the Member States of the European Union". Report from the Commission to the Council and the European Parliament. 12 May 2013. Retrieved 9 July 2015.
- "U.S. primate import statistics for 2014". International Primate Protection League. Retrieved 9 July 2015.
- Kathleen M. Conlee, Erika H. Hoffeld and Martin L. Stephens (2004) Demographic Analysis of Primate Research in the United States, ATLA 32, Supplement 1, 315–22
- St. Fleur, Nicholas (12 June 2015). "U.S. Will Call All Chimps 'Endangered'". The New York Times. Retrieved 9 July 2015.
- Lutz, C; Well, A; Novak, M (2003). "Stereotypic and Self-Injurious Behavior in Rhesus Macaques: A Survey and Retrospective Analysis of Environment and Early Experience". American Journal of Primatology. 60 (1): 1–15. doi:10.1002/ajp.10075. PMID 12766938. S2CID 19980505.
- Chan AW, Chong KY, Martinovich C, Simerly C, Schatten G (2001). "Transgenic monkeys produced by retroviral gene transfer into mature oocytes". Science. 291 (5502): 309–12. Bibcode:2001Sci...291..309C. doi:10.1126/science.291.5502.309. PMID 11209082.
- Yang SH, Cheng PH, Banta H, Piotrowska-Nitsche K, Yang JJ, Cheng EC, Snyder B, Larkin K, Liu J, Orkin J, Fang ZH, Smith Y, Bachevalier J, Zola SM, Li SH, Li XJ, Chan AW (2008). "Towards a transgenic model of Huntington's disease in a non-human primate". Nature. 453 (7197): 921–24. Bibcode:2008Natur.453..921Y. doi:10.1038/nature06975. PMC 2652570. PMID 18488016.
- Emborg ME (2007). "Nonhuman primate models of Parkinson's disease". ILAR Journal. 48 (4): 339–55. doi:10.1093/ilar.48.4.339. PMID 17712221.
- McKie, Robin (2 November 2008). "Ban on primate experiments would be devastating, scientists warn". The Observer. London.
- Invertebrate Animal Resources Archived 25 October 2007 at the Wayback Machine. National Center for Research Resources. ncrr.nih.gov
- "Who's Who of Federal Oversight of Animal Issues". Aesop-project.org. Archived from the original on 22 September 2007.
- Collins FS, Rossant J, Wurst W (2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- Class B dealers Archived 29 April 2010 at the Wayback Machine, Humane Society of the United States.
- "Who's Who of Federal Oversight of Animal Issues" Archived 22 September 2007 at the Wayback Machine, Aesop Project.
- Salinger, Lawrence and Teddlie, Patricia. "Stealing Pets for Research and Profit: The Enforcement (?) of the Animal Welfare Act" Archived 16 January 2013 at archive.today, paper presented at the annual meeting of the American Society of Criminology, Royal York, Toronto, 15 October 2006
- Reitman, Judith (1995) Stolen for Profit, Zebra, ISBN 0-8217-4951-X.
- Moran, Julio (12 September 1991) "Three Sentenced to Prison for Stealing Pets for Research," L.A. Times.
- Francione, Gary. Animals, Property, and the Law. Temple University Press, 1995, p. 192; Magnuson, Warren G., Chairman. "Opening remarks in hearings prior to enactment of Pub. L. 89-544, the Laboratory Animal Welfare Act," U.S. Senate Committee on Commerce, 25 March 1966.
- Notorious Animal Dealer Loses License and Pays Record Fine, The Humane Society of the United States
- Animal Testing: Where Do the Animals Come From?. American Society for the Prevention of Cruelty to Animals. According to the ASPCA, the following states prohibit shelters from providing animals for research: Connecticut, Delaware, Hawaii, Maine, Maryland, Massachusetts, New Hampshire, New Jersey, New York, Pennsylvania, Rhode Island, South Carolina, Vermont, and West Virginia.
- "Council Directive 86/609/EEC of 24 November 1986". Eur-lex.europa.eu.
- "Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes Text with EEA relevance". Eur-lex.europa.eu.
- Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Archived 31 July 2007 at the Wayback Machine Department for Environment, Food and Rural Affairs
- ""Statistics of Scientific Procedures on Living Animals", Statistics of Scientific Procedures on Living Animals, Home Office" (PDF). 2004. p. 87.
- U.S. Primate Imports Spike International Primate Protection League April 2007
- Duncan IJ, Petherick JC (1991). "The implications of cognitive processes for animal welfare". Journal of Animal Science. 69 (12): 5017–22. doi:10.2527/1991.69125017x. PMID 1808195.
- Curtis SE, Stricklin WR (1991). "The importance of animal cognition in agricultural animal production systems: an overview". Journal of Animal Science. 69 (12): 5001–07. doi:10.2527/1991.69125001x. PMID 1808193.
- Carbone, p. 149.
- Rollin drafted the 1985 Health Research Extension Act and an animal welfare amendment to the 1985 Food Security Act: see Rollin, Bernard. "Animal research: a moral science. Talking Point on the use of animals in scientific research", EMBO Reports 8, 6, 2007, pp. 521–25
- Rollin, Bernard. The Unheeded Cry: Animal Consciousness, Animal Pain, and Science. New York: Oxford University Press, 1989, pp. xii, 117–18, cited in Carbone 2004, p. 150.
- Griffin DR, Speck GB (2004). "New evidence of animal consciousness". Animal Cognition. 7 (1): 5–18. doi:10.1007/s10071-003-0203-x. PMID 14658059. S2CID 8650837.
- Allen C (1998). "Assessing animal cognition: ethological and philosophical perspectives". Journal of Animal Science. 76 (1): 42–47. doi:10.2527/1998.76142x. PMID 9464883.
- American Psychological Association. "Dogs' Intelligence on Par With Two-year-old Human, Canine Researcher Says." ScienceDaily, 10 August 2009. <www.sciencedaily.com/releases/2009/08/090810025241.htm>
- "Animal Welfare Act 1999". Parliamentary Counsel Office. 2015. Retrieved 23 January 2016.
- "Norwegian animal welfare act". Michigan State University. 2011. Retrieved 25 January 2016. Cite journal requires
- "Guide for the Care and Use of Laboratory Animals", ILAR, National Research Council, National Academies Press, 1996, p. 64, ISBN 0-309-05377-3.
- "How to Work With Your Institutional Animal Care and Use Committee (IACUC)". ori.hhs.gov.
- Devolder, Katrien; Eggel, Matthias (2019). "No Pain, No Gain? In Defence of Genetically Disenhancing (Most) Research Animals". Animals. 9 (4): 154. doi:10.3390/ani9040154. PMC 6523187. PMID 30970545.
- Flecknell P (2002). "Replacement, reduction and refinement". ALTEX. 19 (2): 73–78. PMID 12098013.
- Animal Procedures Committee: review of cost-benefit assessment in the use of animals in research Archived 27 February 2008 at the Wayback Machine The Animal Procedures Committee, June 2003 p46-7
- Carbone, Larry. "Euthanasia," in Bekoff, M. and Meaney, C. Encyclopedia of Animal Rights and Welfare. Greenwood Publishing Group, pp. 164–66, cited in Carbone 2004, pp. 189–90.
- Cooper, Dale (11 June 2017). ""Euthanasia Guidelines", Research animal resources". University of Minnesota.
- Close B, Banister K, Baumans V, Bernoth EM, Bromage N, Bunyan J, Erhardt W, Flecknell P, Gregory N, Hackbarth H, Morton D, Warwick C (1996). "Recommendations for euthanasia of experimental animals: Part 1". Laboratory Animals. 30 (4): 293–316 (295). doi:10.1258/002367796780739871. PMID 8938617.
- "Guide for the Care and Use of Laboratory Animals", ILAR, National Research Council, National Academies Press, 1996, p. 65, ISBN 0-309-05377-3.
- "AVMA Guidelines on Euthanasia, June 2007 edition, Report of the AVMA Panel on Euthanasia" (PDF). Avma.org. Archived from the original (PDF) on 15 August 2011.
- "Select Committee on Animals in Scientific Procedures Report", House of Lords, 16 July 2002. See chapter 3: "The purpose and nature of animal experiments." Retrieved 6 July 2010.
- Job CK (2003). "Nine-banded armadillo and leprosy research". Indian Journal of Pathology & Microbiology. 46 (4): 541–50. PMID 15025339.
- Venken KJ, Bellen HJ (2005). "Emerging technologies for gene manipulation in Drosophila melanogaster". Nature Reviews Genetics. 6 (3): 167–78. doi:10.1038/nrg1553. PMID 15738961. S2CID 21184903.
- Sung YH, Song J, Lee HW (2004). "Functional genomics approach using mice". Journal of Biochemistry and Molecular Biology. 37 (1): 122–32. doi:10.5483/BMBRep.2004.37.1.122. PMID 14761310.
- Janies D, DeSalle R (1999). "Development, evolution, and corroboration". The Anatomical Record. 257 (1): 6–14. doi:10.1002/(SICI)1097-0185(19990215)257:1<6::AID-AR4>3.0.CO;2-I. PMID 10333399.
- Akam M (1995). "Hox genes and the evolution of diverse body plans". Philosophical Transactions of the Royal Society B. 349 (1329): 313–19. Bibcode:1995RSPTB.349..313A. doi:10.1098/rstb.1995.0119. PMID 8577843.
- Prasad BC, Reed RR (1999). "Chemosensation: Molecular mechanisms in worms and mammals". Trends in Genetics. 15 (4): 150–53. doi:10.1016/S0168-9525(99)01695-9. PMID 10203825.
- Schafer WR (2006). "Neurophysiological methods in C. elegans: an introduction". WormBook: 1–4. doi:10.1895/wormbook.1.113.1. PMC 4780964. PMID 18050439.
- Yamamuro, Yutaka (2006). "Social behavior in laboratory rats: Applications for psycho-neuroethology studies". Animal Science Journal. 77 (4): 386–94. doi:10.1111/j.1740-0929.2006.00363.x.
- Marler P., Slabbekoorn H, Nature's Music: The Science of Birdsong, Academic Press, 2004. ISBN 0-12-473070-1[page needed]
- For example "in addition to providing the chimpanzees with enrichment, the termite mound is also the focal point of a tool-use study being conducted", from the web page of the Lincoln Park Zoo. Retrieved 25 April 2007.
- Festing, M., "Inbred Strains of Mice and their Characteristics", The Jackson Laboratory . Retrieved 30 January 2008.
- Peichel CL (2005). "Fishing for the secrets of vertebrate evolution in threespine sticklebacks". Developmental Dynamics. 234 (4): 815–23. doi:10.1002/dvdy.20564. PMID 16252286.
- Peichel CL, Nereng KS, Ohgi KA, Cole BL, Colosimo PF, Buerkle CA, Schluter D, Kingsley DM (2001). "The genetic architecture of divergence between threespine stickleback species" (PDF). Nature. 414 (6866): 901–05. Bibcode:2001Natur.414..901P. doi:10.1038/414901a. PMID 11780061. S2CID 4304296.
- Ramaswamy S, McBride JL, Kordower JH (2007). "Animal models of Huntington's disease". ILAR Journal. 48 (4): 356–73. doi:10.1093/ilar.48.4.356. PMID 17712222.
- Rees DA, Alcolado JC (2005). "Animal models of diabetes mellitus". Diabetic Medicine. 22 (4): 359–70. doi:10.1111/j.1464-5491.2005.01499.x. PMID 15787657.
- Iwakuma T, Lozano G (2007). "Crippling p53 activities via knock-in mutations in mouse models". Oncogene. 26 (15): 2177–84. doi:10.1038/sj.onc.1210278. PMID 17401426.
- Frese KK, Tuveson DA (2007). "Maximizing mouse cancer models". Nature Reviews Cancer. 7 (9): 645–58. doi:10.1038/nrc2192. PMID 17687385. S2CID 6490409.
- Dunham SP (2006). "Lessons from the cat: development of vaccines against lentiviruses". Veterinary Immunology and Immunopathology. 112 (1–2): 67–77. doi:10.1016/j.vetimm.2006.03.013. PMID 16678276.
- Vail DM, MacEwen EG (2000). "Spontaneously occurring tumors of companion animals as models for human cancer". Cancer Investigation. 18 (8): 781–92. doi:10.3109/07357900009012210. PMID 11107448. S2CID 32489790.
- Tolwani RJ, Jakowec MW, Petzinger GM, Green S, Waggie K (1999). "Experimental models of Parkinson's disease: insights from many models". Laboratory Animal Science. 49 (4): 363–71. PMID 10480640.
- Pound P, Ebrahim S, Sandercock P, Bracken MB, Roberts I (2004). Reviewing Animal Trials Systematically (RATS) Group. "Where is the evidence that animal research benefits humans?". BMJ. 328 (7438): 514–47. doi:10.1136/bmj.328.7438.514. PMC 351856. PMID 14988196.
- Langley, Gill (2006) next of kin...A report on the use of primates in experiments Archived 27 February 2008 at the Wayback Machine, BUAV.
- The History of Deep Brain Stimulation Archived 31 March 2017 at the Wayback Machine. parkinsonsappeal.com
- Platt JL, Lin SS (1998). "The future promises of xenotransplantation". Annals of the New York Academy of Sciences. 862 (1): 5–18. Bibcode:1998NYASA.862....5P. doi:10.1111/j.1749-6632.1998.tb09112.x. PMID 9928201.
- Schuurman HJ, Pierson RN (2008). "Progress towards clinical xenotransplantation". Frontiers in Bioscience. 13 (13): 204–20. doi:10.2741/2671. PMID 17981539.
- Valdés-González RA, Dorantes LM, Garibay GN, Bracho-Blanchet E, Mendez AJ, Dávila-Pérez R, Elliott RB, Terán L, White DJ (2005). "Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: a 4-year study". European Journal of Endocrinology. 153 (3): 419–27. doi:10.1530/eje.1.01982. PMID 16131605.
- Valdés-González RA, White DJ, Dorantes LM, Terán L, Garibay-Nieto GN, Bracho-Blanchet E, Dávila-Pérez R, Evia-Viscarra L, Ormsby CE, Ayala-Sumuano JT, Silva-Torres ML, Ramírez-González B (2007). "Three-yr follow-up of a type 1 diabetes mellitus patient with an islet xenotransplant". Clinical Transplantation. 21 (3): 352–57. doi:10.1111/j.1399-0012.2007.00648.x. PMID 17488384. S2CID 22668776.
- Townsend, Mark (20 April 2003). "Exposed: secrets of the animal organ lab" Archived 6 July 2008 at the Wayback Machine, The Guardian.
- Curtis, Polly (11 July 2003). "Home Office under renewed fire in animal rights row", The Guardian.
- Household Product Tests Archived 27 February 2008 at the Wayback Machine BUAV
- Fifth Report on the Statistics on the Number of Animals used for Experimental and other Scientific Purposes in the Member States of the European Union, Commission of the European Communities, published November 2007
- Abbott A (2005). "Animal testing: More than a cosmetic change" (PDF). Nature. 438 (7065): 144–46. Bibcode:2005Natur.438..144A. doi:10.1038/438144a. PMID 16281001. S2CID 4422086. Archived from the original (PDF) on 27 February 2008.
- Watkins JB (1989). "Exposure of rats to inhalational anesthetics alters the hepatobiliary clearance of cholephilic xenobiotics". The Journal of Pharmacology and Experimental Therapeutics. 250 (2): 421–27. PMID 2760837.
- Watt JA, Dickinson RG (1990). "The effect of diethyl ether, pentobarbitone and urethane anaesthesia on diflunisal conjugation and disposition in rats". Xenobiotica. 20 (3): 289–301. doi:10.3109/00498259009046848. PMID 2336839.
- Walum E (1998). "Acute oral toxicity". Environmental Health Perspectives. 106 (Suppl 2): 497–503. doi:10.2307/3433801. JSTOR 3433801. PMC 1533392. PMID 9599698.
- Inter-Governmental Organization Eliminates the LD50 Test, The Humane Society of the United States (2003-02-05)
- "OECD guideline 405, Organisation for Economic Co-operation and Development" (PDF). Archived from the original (PDF) on 27 February 2008. Retrieved 6 April 2015.
- Species Used in Research: Rabbit, Humane Society of the United States
- Wilhelmus KR (2001). "The Draize eye test". Survey of Ophthalmology. 45 (6): 493–515. doi:10.1016/S0039-6257(01)00211-9. PMID 11425356.
- Secchi A, Deligianni V (2006). "Ocular toxicology: the Draize eye test". Current Opinion in Allergy and Clinical Immunology. 6 (5): 367–72. doi:10.1097/01.all.0000244798.26110.00. PMID 16954791. S2CID 24972694.
- Draize rabbit eye test replacement milestone welcomed. Dr Hadwen Trust (2009-09-21)
- Toxicity Testing for Assessment of Environmental Agents" National Academies Press, (2006), p. 21.
- Hartung T (2009). "Toxicology for the twenty-first century". Nature. 460 (7252): 208–12. Bibcode:2009Natur.460..208H. doi:10.1038/460208a. PMID 19587762. S2CID 851143.
- "Where is the toxicology for the twenty-first century?". Pro-Test Italia. 2013. Retrieved 30 January 2014.
- Smith LL (2001). "Key challenges for toxicologists in the 21st century". Trends Pharmacol. Sci. 22 (6): 281–85. doi:10.1016/S0165-6147(00)01714-4. PMID 11395155.
- Brown SL, Brett SM, Gough M, Rodricks JV, Tardiff RG, Turnbull D (1988). "Review of interspecies risk comparisons". Regul. Toxicol. Pharmacol. 8 (2): 191–206. doi:10.1016/0273-2300(88)90028-1. PMID 3051142.
- Burden, N; Sewell, F; Chapman, K (2015). "Testing Chemical Safety: What Is Needed to Ensure the Widespread Application of Non-animal Approaches?". PLOS Biol. 13 (5): e1002156. doi:10.1371/journal.pbio.1002156. PMC 4446337. PMID 26018957.
- Moczko, E; Mirkes, EM; Cáceres, C; Gorban, AN; Piletsky, S (2016). "Fluorescence-based assay as a new screening tool for toxic chemicals". Scientific Reports. 6: 33922. Bibcode:2016NatSR...633922M. doi:10.1038/srep33922. PMC 5031998. PMID 27653274.
- Stephens, Martin & Rowan, Andrew. An overview of Animal Testing Issues, Humane Society of the United States
- "Cosmetics animal testing in the EU".
- Engebretson, Monica (16 March 2014). "India Joins the EU and Israel in Surpassing the US in Cruelty-Free Cosmetics Testing Policy". The World Post.
- "Cruelty Free International Applauds Congressman Jim Moran for Bill to End Cosmetics Testing on Animals in the United States" (Press release). 5 March 2014. Archived from the original on 18 March 2014.
- Fox, Stacy (10 March 2014). "Animal Attraction: Federal Bill to End Cosmetics Testing on Animals Introduced in Congress" (Press release). Humane Society of the United States. Archived from the original on 11 March 2014.
- Osborn, Andrew & Gentleman, Amelia."Secret French move to block animal-testing ban", The Guardian (19 August 2003). Retrieved 27 February 2008.
- Mohan, Vishwa (14 October 2014). "India bans import of cosmetics tested on animals". The Times of India. Retrieved 14 October 2014.
- "EU Directive 2001/83/EC, p. 44". Eur-lex.europa.eu.
- "EU Directive 2001/83/EC, p. 45". Eur-lex.europa.eu.
- Patronek, G; Rauch, A (1 January 2007). "Systematic review of comparative studies examining alternatives to the harmful use of animals in biomedical education". Journal of the American Veterinary Medical Association. 230 (1): 37–43. doi:10.2460/javma.230.1.37. PMID 17199490.
- Hart, L; Hart, B; Wood, M (2008). Why Dissection: Animal Use in Education. Westport: Greenwood Press. ISBN 978-0-313-32390-4.
- Orlans, Barbara; Beauchamp, Tom; Dresser, Rebecca; Morton, David; Gluck, John (1998). The Human Use of Animals. Oxford University Press. pp. 213. ISBN 978-0-19-511908-4.
- Downey, Maureen (25 June 2013). "Should students dissect animals or should schools move to virtual dissections?". Atlanta Journal Constitution. Retrieved 7 July 2015.
- Pulla, Priyanka (6 August 2014). "Dissections banned in Indian universities". Science. Retrieved 7 July 2015.
- Shine, Nicole. "The Battle Over High School Animal Dissection". Pacific Standard. Retrieved 7 July 2015.
- "Invertebrates in Education and Conservation Conference | Department of Neuroscience". Neurosci.arizona.edu. Archived from the original on 28 July 2020. Retrieved 6 April 2015.
- Dalal, Rooshin; Even, Megha; Sandusky, Chad; Barnard, Neal (August 2005). "Replacement Alternatives in Education: Animal-Free Teaching" (Abstract from Fifth World Congress on Alternatives and Animal Use in the Life Sciences, Berlin). The Physicians Committee for Responsible Medicine. Archived from the original on 22 July 2014. Retrieved 9 April 2015.
- "The NORINA database of alternatives". Oslovet.norecopa.no. Retrieved 6 April 2015.
- "Welcome". Interniche.org. Retrieved 6 April 2015.
- "Row over US mobile phone 'cockroach backpack' app". BBC News. 9 November 2013. Retrieved 9 November 2013.
- Hamilton, Anita (1 November 2013). "Resistance is Futile: PETA Attempts to Halt the Sale of Remote-Controlled Cyborg Cockroaches". Time. Retrieved 10 November 2013.
- Brook, Tom Vanden, "Brain Study, Animal Rights Collide", USA Today (7 April 2009), p. 1.
- Kelly, Jon (7 March 2013). "Who, What, Why: Does shooting goats save soldiers' lives?". BBC News Magazine.
- Londoño, Ernesto (24 February 2013). "Military is required to justify using animals in medic training after pressure from activists". The Washington Post. Archived from the original on 15 December 2013.
- Vergakis, Brock (14 February 2014). "Coast Guard reduces use of live animals in training". Retrieved 7 July 2015.
- Bender, Bryan (12 November 2014). "Military to curtail use of live animals in medical training". Boston Globe. Retrieved 7 July 2015.
- Champaco, Brent (15 August 2013). "PETA: Madigan Army Medical Center Has Stopped 'Cruel' Ferret-Testing". Patch. Retrieved 7 July 2015.
- Rollin BE (2006). "The regulation of animal research and the emergence of animal ethics: A conceptual history" (PDF). Theoretical Medicine and Bioethics. 27 (4): 285–304. doi:10.1007/s11017-006-9007-8. PMID 16937023. S2CID 18620094.
- Riffkin, Rebecca (18 May 2015). "In U.S., More Say Animals Should Have Same Rights as People". Gallup. Retrieved 7 July 2015.
- Funk, Cary; Rainie, Lee (29 January 2015). "Public and Scientists' Views on Science and Society". Pew Research Center. Retrieved 7 July 2015.
- Singer, Peter (ed.). "A Companion to Ethics". Blackwell Companions to Philosophy, 1991.
- Chapter 14, Discussion of ethical issues, p . 244 Archived 28 September 2011 at the Wayback Machine in: The ethics of research involving animals Archived 29 April 2011 at the Wayback Machine at the Nuffield Council on Bioethics. Published 25 May 2005
- George, Roger. "Donald Watson 2002 Unabridged Interview" (PDF). Archived (PDF) from the original on 27 October 2019.
- Rollin, Bernard E. (1998) "The moral status of animals and their use as experimental subjects," in Kuhse, Helga and Singer, Peter (eds.). "A Companion to Bioethics". Blackwell Publishing, ISBN 0-631-23019-X.
- Bebarta V, Luyten D, Heard K (2003). "Emergency medicine animal research: does use of randomization and blinding affect the results?". Academic Emergency Medicine. 10 (6): 684–87. doi:10.1111/j.1553-2712.2003.tb00056.x. PMID 12782533.
- Macleod, Malcolm R.; van der Worp, H. Bart; Sena, Emily S.; Howells, David W.; Dirnagl, Ulrich; Donnan, Geoffrey A. (2008). "Evidence for the efficacy of NXY-059 in experimental focal cerebral ischaemia is confounded by study quality". Stroke. 39 (10): 2824–29. doi:10.1161/strokeaha.108.515957. PMID 18635842.
- Sena E, Wheble P, Sandercock P, Macleod M (2007). "Systematic review and meta-analysis of the efficacy of tirilazad in experimental stroke". Stroke. 38 (2): 388–94. doi:10.1161/01.str.0000254462.75851.22. PMID 17204689.
- Hirst JA, Howick J, Aronson J, Roberts N, Perera R, Koshiaris C, Heneghan C (2014). "The Need for Randomization in Animal Trials: An Overview of Systematic Reviews". PLOS One. 9 (6): e98856. Bibcode:2014PLoSO...998856H. doi:10.1371/journal.pone.0098856. PMC 4048216. PMID 24906117.
- Van der Worp B, Sena E, Porritt M, Rewell S, O'Collins V, Macleod MR (2010). "Can Animal Models of Disease Reliably Inform Human Studies?". PLOS Med. 7 (3): e1000245. doi:10.1371/journal.pmed.1000245. PMC 2846855. PMID 20361020.
- Gagneux P, Moore JJ, Varki A (2005). "The ethics of research on great apes". Nature. 437 (7055): 27–29. Bibcode:2005Natur.437...27G. doi:10.1038/437027a. PMID 16136111. S2CID 11500691.
- Vermij P (2003). "Europe's last research chimps to retire". Nature Medicine. 9 (8): 981. doi:10.1038/nm0803-981b. PMID 12894144. S2CID 9892510.
- St Fleur, Nicholas (12 June 2015). "U.S. Will Call All Chimps 'Endangered'". The New York Times. Retrieved 7 July 2015.
- Kaiser, Jocelyn (26 June 2013). "NIH Will Retire Most Research Chimps, End Many Projects". sciencemag.org. Retrieved 7 July 2015.
- "Summary of House of Lords Select Committee on Animals in Scientific Procedures". UK Parliament. 24 July 2002. Retrieved 13 July 2012.
- 韓国・食薬庁で「実験動物慰霊祭」挙行 Archived 29 August 2007 at the Wayback Machine
- Huxley AF, Simmons RM (1971). "Proposed Mechanism of Force Generation in Striated Muscle". Nature. 233 (5321): 533–38. Bibcode:1971Natur.233..533H. doi:10.1038/233533a0. PMID 4939977. S2CID 26159256.
- Gordon AM, Huxley AF, Julian FJ (1966). "The variation in isometric tension with sarcomere length in vertebrate muscle fibres". The Journal of Physiology. 184 (1): 170–92. doi:10.1113/jphysiol.1966.sp007909. PMC 1357553. PMID 5921536.
- Ford LE, Huxley AF, Simmons RM (1985). "Tension transients during steady shortening of frog muscle fibres". The Journal of Physiology. 361 (1): 131–50. doi:10.1113/jphysiol.1985.sp015637. PMC 1192851. PMID 3872938.
- Lutz GJ, Lieber RL (2000). "Myosin isoforms in anuran skeletal muscle: Their influence on contractile properties and in vivo muscle function". Microscopy Research and Technique. 50 (6): 443–57. doi:10.1002/1097-0029(20000915)50:6<443::AID-JEMT3>3.0.CO;2-5. PMID 10998635.
- Liber, R. L. (2002). Skeletal Muscle Structure, Function, and Plasticity: The Physiological Basis of Rehabilitation, 2nd ed. Lippincott Williams & Wilkins, ISBN 978-0-7817-3061-7.
- Franklin, Ben A. (30 August 1987) "Going to Extremes for 'Animal Rights'", The New York Times.
- Holden C (1986). "A pivotal year for lab animal welfare". Science. 232 (4747): 147–50. Bibcode:1986Sci...232..147H. doi:10.1126/science.3952503. PMID 3952503.
- Laville, Sandra (8 February 2005). "Lab monkeys 'scream with fear' in tests", The Guardian.
- "Columbia in animal cruelty dispute", CNN (2003-10-12)
- Benz, Kathy and McManus, Michael (17 May 2005). PETA accuses lab of animal cruelty, CNN.
- Scott, Luci (1 April 2006). "Probe leads to Covance fine", The Arizona Republic. Retrieved 8 March 2021.
- Huggett B (2008). "When animal rights turns ugly". Nature Biotechnology. 26 (6): 603–05. doi:10.1038/nbt0608-603. PMID 18536673. S2CID 8006958.
- Malone BJ, Kumar VR, Ringach DL (2007). "Dynamics of Receptive Field Size in Primary Visual Cortex". Journal of Neurophysiology. 97 (1): 407–14. CiteSeerX 10.1.1.133.3969. doi:10.1152/jn.00830.2006. PMID 17021020.
- Epstein, David (22 August 2006). Throwing in the Towel, Inside Higher Education
- Predators Unleashed, Investor's Business Daily (2006-08-24)
- McDonald, Patrick Range (8 August 2007). UCLA Monkey Madness, LA Weekly.
- "It's a Dog's Life," Countryside Undercover, Channel Four Television, UK (26 March 1997).
- "It's a dog's life" Archived 8 March 2012 at the Wayback Machine, Small World Productions (2005). Retrieved 6 July 2010.
- "A controversial laboratory". BBC News. 18 January 2001. Retrieved 13 July 2012.
- Broughton, Zoe (March 2001). "Seeing Is Believing – cruelty to dogs at Huntingdon Life Sciences", The Ecologist.
- "From push to shove", Southern Poverty Law Group Intelligence Report, Fall 2002
- Lewis, John E. "Statement of John Lewis", US Senate Committee on Environment and Public Works, 26 October 2005, accessed 17 January 2011.
- Evers, Marco. "Resisting the Animal Avengers", Part 1, Part 2, Der Spiegel, 19 November 2007.
- Weaver, Matthew. "Animal rights activists jailed for terrorising suppliers to Huntingdon Life Sciences", The Guardian, 25 October 2010.
- Herbert, Ian (27 January 2007). "Collapse in support for animal rights extremist attacks", The Independent.
- Knight, Andrew (May 2008). "Systematic reviews of animal experiments demonstrate poor contributions toward human healthcare". Reviews on Recent Clinical Trials. 3 (2): 89–96. doi:10.2174/157488708784223844. ISSN 1574-8871. PMID 18474018.
- Greek, Ray; Menache, Andre (11 January 2013). "Systematic Reviews of Animal Models: Methodology versus Epistemology". International Journal of Medical Sciences. 10 (3): 206–21. doi:10.7150/ijms.5529. ISSN 1449-1907. PMC 3558708. PMID 23372426.
- Bracken, Michael B (1 March 2009). "Why animal studies are often poor predictors of human reactions to exposure". Journal of the Royal Society of Medicine. 102 (3): 120–22. doi:10.1258/jrsm.2008.08k033. ISSN 0141-0768. PMC 2746847. PMID 19297654.
- Pound, Pandora; Ebrahim, Shah; Sandercock, Peter; Bracken, Michael B; Roberts, Ian (28 February 2004). "Where is the evidence that animal research benefits humans?". BMJ : British Medical Journal. 328 (7438): 514–17. doi:10.1136/bmj.328.7438.514. ISSN 0959-8138. PMC 351856. PMID 14988196.
- Perel, Pablo; Roberts, Ian; Sena, Emily; Wheble, Philipa; Briscoe, Catherine; Sandercock, Peter; Macleod, Malcolm; Mignini, Luciano E.; Jayaram, Pradeep; Khan, Khalid S. (25 January 2007). "Comparison of treatment effects between animal experiments and clinical trials: systematic review". BMJ. 334 (7586): 197. doi:10.1136/bmj.39048.407928.BE. ISSN 0959-8138. PMC 1781970. PMID 17175568.
- Schulz, Kenneth F.; Chalmers, Iain; Altman, Douglas G. (5 February 2002). "The Landscape and Lexicon of Blinding in Randomized Trials". Annals of Internal Medicine. 136 (3): 254–59. doi:10.7326/0003-4819-136-3-200202050-00022. ISSN 0003-4819. PMID 11827510. S2CID 34932997.
- "What are the 3Rs?". NC3Rs. Archived from the original on 1 August 2014. Retrieved 16 December 2018.
- Kolar R (2002). "ECVAM: desperately needed or superfluous? An animal welfare perspective". Altern Lab Anim. 30 (Suppl 2): 169–74. doi:10.1177/026119290203002S26. PMID 12513669.
- Schuppli CA, Fraser D, McDonald M (2004). "Expanding the three Rs to meet new challenges in humane animal experimentation". Altern Lab Anim. 32 (5): 525–32. doi:10.1177/026119290403200507. PMID 15656775. S2CID 25015151.
- Rusche B (2003). "The 3Rs and animal welfare – conflict or the way forward?". ALTEX. 20 (Suppl 1): 63–76. PMID 14671703.
- Alexander FA, Eggert S, Wiest J (February 2018). "Skin-on-a-Chip: Transepithelial Electrical Resistance and Extracellular Acidification Measurements through an Automated Air-Liquid Interface". Genes. 9 (2): 114. doi:10.3390/genes9020114. PMC 5852610. PMID 29466319.
- "Alternatives to Animal Testing | Animals Used for Experimentation | The Issues". Peta.org. Retrieved 6 April 2015.
- Rhodes, Margaret (28 May 2015). "Inside L'Oreal's Plan to 3-D Print Human Skin". Wired. Retrieved 7 July 2015.
- Watts, Geoff (27 January 2007). "Alternatives to animal experimentation". BMJ. 334 (7586): 182–84. doi:10.1136/bmj.39058.469491.68. PMC 1782004. PMID 17255608.
- Edelman, L; Eddy, J; Price, N (July–August 2010). "In silico models of cancer". Wiley Interdiscip Rev Syst Biol Med. 2 (4): 438–59. doi:10.1002/wsbm.75. PMC 3157287. PMID 20836040.
- "Microdosing". 3Rs. Canadian Council on Animal Care in Science. Archived from the original on 7 June 2015. Retrieved 7 July 2015.
- "What Is A PET Scan? How Does A PET Scan Work?". Medicalnewstoday.com.
- Jiang J, Liu B, Nasca PC, Han W, Zou X, Zeng X, Tian X, Wu Y, Zhao P, Li J (2009). "Comparative study of control selection in a national population -based case-control study: Estimating risk of smoking on cancer deaths in Chinese men". International Journal of Medical Sciences. 6 (6): 329–37. doi:10.7150/ijms.6.329. PMC 2777271. PMID 19918375.
- McNeil, Donald (13 January 2014). "PETA's Donation to Help Save Lives, Animal and Human". The New York Times. Retrieved 7 July 2015.
- Bernstein, Fred (4 October 2005). "An On-Screen Alternative to Hands-On Dissection". The New York Times. Retrieved 7 July 2015.
- "NTP Interagency Center for the Evaluation of Alternative Toxicological Methods – NTP". Iccvam.niehs.nih.gov. Archived from the original on 9 December 2013. Retrieved 6 April 2015.
- ZEBET database on alternatives to animal experiments on the Internet (AnimAlt-ZEBET). BfR (30 September 2004). Retrieved on 2013-01-21.
- About JaCVAM-Organization of JaCVAM Archived 11 May 2012 at the Wayback Machine. Jacvam.jp. Retrieved on 2013-01-21.
- EPAA – Home Archived 1 November 2013 at the Wayback Machine. Ec.europa.eu. Retrieved on 2013-01-21.
- ecopa – european consensus-platform for alternatives. Ecopa.eu. Retrieved on 2013-01-21.
- Center for Alternatives to Animal Testing – Johns Hopkins Bloomberg School of Public Health. Caat.jhsph.edu. Retrieved on 2013-01-21.
- "NC3Rs". NC3Rs.org.uk. Retrieved 6 April 2015.
- Works cited
- Carbone, Larry. (2004). What animals want : expertise and advocacy in laboratory animal welfare policy. Oxford: Oxford University Press. ISBN 978-0199721887. OCLC 57138138.
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