Best known for his portrayal of Rob Titchener in BBC Radio 4's soap opera The Archers, he has also played the role of the Maitre d’ of the Palm Court Restaurant, Mr Perez, in Mr Selfridge. Watson voiced the characters of both James Bond and Auric Goldfinger in the 2012 video game 007 Legends based on the James Bond movies. Watson also voiced the characters Mumkhar and Metal Face in the video game Xenoblade Chronicles. Watson appeared in the National Theatre's production of The Beaux' Stratagem.
In 2015, the audience at the Radio Times Festival greeted Watson by booing in response to the plotline involving his character Rob Titchener in The Archers. He later told The Observer's Vanessa Thorpe, "I have now removed all social media from my house because some of the things people said were quite awful. […] The strength of feeling took me by surprise. And then it starts to impact on your own personal life. I would rather not see it."
In February 2017, Watson participated in live interviews to answer BBC Radio 4 listeners' questions about his experience of acting in The Archers. The following year, in a series of Halloween-themed radio adaptations for BBC Radio 4's 15 Minute Drama programme based on the second book in the Pan Book of Horror Stories series, Watson played the part of Sbirro in Stanley Ellin's short story The Speciality of the House and the title character in Carl Stephenson's Leiningen Versus the Ants.
In 2016, Watson told Radio Times' David Brown, "I support Compassion in World Farming. I hate mega dairies, they're appalling things. I've got no interest in country pursuits, I've never held a cricket bat, even though as Rob I've managed to cheat during the single wickets in Ambridge. And I hate hunting. I would probably have been standing with the hunt saboteurs instead of falsely accusing one of assaulting me. So the real me probably wouldn't fare too well in Ambridge."
- "BBC Radio 4 The Archers: Rob Titchener – Timothy Watson". BBC. Retrieved 3 April 2016.
- "Xenoblade Chronicles". Behind The Voice Actors. Retrieved 9 January 2019.
- "Timothy Watson". Royal National Theatre. March 2015. Retrieved 3 April 2016.[permanent dead link]
- Daoust, Phil (6 July 2014). "Pick of the day". The Guardian. London. Retrieved 4 April 2016.
- Furness, Hannah (26 September 2015). "Archers villain has to delete social media". The Daily Telegraph. London. Retrieved 5 February 2017.
- Thorpe, Vanessa (26 September 2015). "Actor who plays Archers villain in shock at social media onslaught". The Observer. London. Retrieved 4 April 2016.
- "13 things we learned from our live interview with Timothy Watson aka Rob Titchener". BBC. 3 February 2017. Retrieved 5 February 2017.
- Presenter: Roger Bolton; Producer: Katherine Godfrey (24 January 2017). "Radio 2 Schedule Changes". Feedback. 16:48 minutes in. BBC. BBC Radio 4. Retrieved 24 February 2017.
- Costain: Samuel Barnett; Vincent: Kenneth Collard; Sbirro: Timothy Watson; Haysou: Adam James; Writer: Stanley Ellin; Adapted by: Anita Sullivan; Director & Producer: Karen Rose (30 October 2018). "The Second Pan Book of Horror Stories: The Speciality of the House". 15 Minute Drama. BBC. BBC Radio 4. Retrieved 1 November 2018.
- Leiningren: Timothy Watson; Rica: Kathryn Drysdale; Luiz: Adam James; Writer: Carl Stephenson; Adapted by: Anita Sullivan; Director & Producer: Karen Rose (1 November 2018). "The Second Pan Book of Horror Stories: Leiningen Versus The Ants". 15 Minute Drama. BBC. BBC Radio 4. Retrieved 1 November 2018.
- Brown, David (29 March 2016). "The Archers: "I'm evil personified," says Rob Titchener actor Timothy Watson". Radio Times. London. Retrieved 5 February 2017.