- Melbourne, Australia (1985)
- Hangzhou, China (1982)
- Strasbourg, France (1960)
- Sekondi-Takoradi, Ghana (2001)
- Belfast, Northern Ireland, United Kingdom (2014)
- Padua, Italy (1983)
- Kyoto, Japan (1959)
- Barcelona, Spain (1980)
- Taipei, Taiwan (1996)
- Praia, Cape Verde (2015)
Boston has friendship or partnership relationships with three additional cities:
- "Boston Sister Cities". The City of Boston. Archived from the original on 2011-08-24. Retrieved 2013-12-31.
- "Friendship treaty Haifa - Boston signed on 10 November 1999" (in Hebrew). Haifa Municipality. Retrieved 2014-01-26.
- Press Office of the Municipality of Valladolid (2007-09-18). "Valladolid and Boston have signed a protocol of friendship between the two cities" (in Spanish). Ayuntamiento de Valladolid. Retrieved 2013-12-31.
- "Partnership of the Historic Bostons". Partnership of the Historic Bostons. Retrieved 2013-12-31.
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