Santa María la Antigua del Darién, formerly also known as Dariena, was a Spanish colonial town founded in 1510 by Vasco Núñez de Balboa, located in present-day Colombia approximately 40 miles south of Acandí, within the municipality of Unguía in the Chocó Department. It was the first city founded by conquistadors in mainland America. After Pascual de Andagoya, a Spanish-Basque conquistador under the direction of Panama governor Pedrarias Dávila, founded Panama City in 1519, Santa María la Antigua del Darién was abandoned and in 1524 was attacked and burned by the indigenous people.
- D'Anghiera, Peter Martyr. De Orbo Novo (in Latin). Trans. Richard Eden as The decades of the newe worlde or west India conteynyng the nauigations and conquestes of the Spanyardes with the particular description of the moste ryche and large landes and Ilands lately founde in the west Ocean perteynyng to the inheritaunce of the kinges of Spayne, Book III, §3. William Powell (London), 1555.
- Vignolo, Paolo (April 2008). "Santa María de la Antigua: Prácticas y representaciones de un culto mariano entre Sevilla y el Darién". e-misférica. Retrieved 10 February 2012.
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