Clostridium is a genus of Gram-positive bacteria. This genus includes several significant human pathogens, including the causative agents of botulism and tetanus. The genus formerly included an important cause of diarrhea, Clostridioides difficile, which was separated after 16S rRNA analysis. They are obligate anaerobes capable of producing endospores. The normal, reproducing cells of Clostridium, called the vegetative form, are rod-shaped, which gives them their name, from the Greek κλωστήρ or spindle. Clostridium endospores have a distinct bowling pin or bottle shape, distinguishing them from other bacterial endospores, which are usually ovoid in shape. Clostridium species inhabit soils and the intestinal tract of animals, including humans. Clostridium is a normal inhabitant of the healthy lower reproductive tract of females.
Clostridium cluster XIVa and Clostridium cluster IV efficiently ferment plant polysaccharide composing dietary fiber, making them important and abundant taxa in the rumen and the human large intestine. Despite the naming, these clusters encompass many bacteria outside the genus Clostridium.
- Clostridium botulinum can produce botulinum toxin in food or wounds and can cause botulism. This same toxin is known as Botox and is used in cosmetic surgery to paralyze facial muscles to reduce the signs of aging; it also has numerous other therapeutic uses.
- Clostridium perfringens causes a wide range of symptoms, from food poisoning to cellulitis, fasciitis, necrotic enteritis and gas gangrene.
- Clostridium tetani causes tetanus.
- Clostridium sordellii can cause a fatal infection in exceptionally rare cases after medical abortions.
Clostridium and Bacillus are both in the phylum Firmicutes, but they are in different classes, orders, and families. Microbiologists distinguish Clostridium from Bacillus by the following features:
- Clostridium grows in anaerobic conditions; Bacillus grows in aerobic conditions.
- Clostridium forms bottle-shaped endospores; Bacillus forms oblong endospores.
- Clostridium does not form the enzyme catalase; Bacillus secretes catalase to destroy toxic byproducts of oxygen metabolism.
Clostridium and Desulfotomaculum are both in the class Clostridia and order Clostridiales, and they both produce bottle-shaped endospores, but they are in different families. Clostridium can be distinguished from Desulfotomaculum on the basis of the nutrients each genus uses (the latter requires sulfur).
The Schaeffer-Fulton stain (0.5% malachite green in water) can be used to distinguish endospores of Bacillus and Clostridium from other microorganisms. There is a commercially available polymerase chain reaction (PCR) test kit (Bactotype) for the detection of C. perfringens and other pathogenic bacteria.
In general, the treatment of clostridial infection is high-dose penicillin G, to which the organism has remained susceptible. Clostridium welchii and Clostridium tetani respond to sulfonamides. Clostridia are also susceptible to tetracyclines, carbapenems (imipenem), metronidazole, vancomycin, and chloramphenicol.
The vegetative cells of clostridia are heat-labile and are killed by short heating at temperatures above 72–75 °C. The thermal destruction of Clostridium spores requires higher temperatures (above 121.1 °C, for example in an autoclave) and longer cooking times (20 min, with a few exceptional cases of > 50 min recorded in the literature). Clostridia and Bacilli are quite radiation-resistant, requiring doses of about 30 kGy, which is a serious obstacle to the development of shelf-stable irradiated foods for general use in the retail market. The addition of lysozyme, nitrate, nitrite and propionic acid salts inhibits clostridia in various foods.
Fructooligosaccharides (fructans) such as inulin, occurring in relatively large amounts in a number of foods such as chicory, garlic, onion, leek, artichoke, and asparagus, have a prebiotic or bifidogenic effect, selectively promoting the growth and metabolism of beneficial bacteria in the colon, such as bifidobacteria and lactobacilli, while inhibiting harmful ones, such as clostridia, fusobacteria, and bacteroides.
In the late 1700s, Germany experienced a number of outbreaks of an illness that seemed connected to eating certain sausages. In 1817, the German neurologist Justinus Kerner detected rod-shaped cells in his investigations into this so-called sausage poisoning. In 1897, the Belgian biology professor Emile van Ermengem published his finding of an endospore-forming organism he isolated from spoiled ham. Biologists classified van Ermengem's discovery along with other known gram-positive spore formers in the genus Bacillus. This classification presented problems, however, because the isolate grew only in anaerobic conditions, but Bacillus grew well in oxygen.
Circa 1880, in the course of studying fermentation and butyric acid synthesis, a scientist surnamed Prazmowski first assigned a binomial name to Clostridium butyricum.:107–108 The mechanisms of anaerobic respiration were still not yet well elucidated at that time,:107–108 so taxonomy of anaerobes was still nascent.
In 1924, Ida A. Bengtson separated van Ermengem's microorganisms from the Bacillus group and assigned them to the genus Clostridium. By Bengtson's classification scheme, Clostridium contained all of the anaerobic endospore-forming rod-shaped bacteria, except the genus Desulfotomaculum.
- Clostridium thermocellum can use lignocellulosic waste and generate ethanol, thus making it a possible candidate for use in production of ethanol fuel. It also has no oxygen requirement and is thermophilic, which reduces cooling cost.
- Clostridium acetobutylicum was first used by Chaim Weizmann to produce acetone and biobutanol from starch in 1916 for the production of cordite (smokeless gunpowder).
- Clostridium botulinum produces a potentially lethal neurotoxin used in a diluted form in the drug Botox, which is carefully injected to nerves in the face, which prevents the movement of the expressive muscles of the forehead, to delay the wrinkling effect of aging. It is also used to treat spasmodic torticollis and provides relief for around 12 to 16 weeks.
- Clostridium butyricum MIYAIRI 588 strain is marketed in Japan, Korea, and China for Clostridium difficile prophylaxis due to its reported ability to interfere with the growth of the latter.
- Clostridium histolyticum has been used as a source of the enzyme collagenase, which degrades animal tissue. Clostridium species excrete collagenase to eat through tissue and, thus, help the pathogen spread throughout the body. The medical profession uses collagenase for the same reason in the débridement of infected wounds. Hyaluronidase, deoxyribonuclease, lecithinase, leukocidin, protease, lipase, and hemolysin are also produced by some clostridia that cause gas gangrene.
- Clostridium ljungdahlii, recently discovered in commercial chicken wastes, can produce ethanol from single-carbon sources including synthesis gas, a mixture of carbon monoxide and hydrogen, that can be generated from the partial combustion of either fossil fuels or biomass.
- Clostridium butyricum converts glycerol to 1,3-propanediol.
- Genes from Clostridium thermocellum have been inserted into transgenic mice to allow the production of endoglucanase. The experiment was intended to learn more about how the digestive capacity of monogastric animals could be improved.
- Nonpathogenic strains of Clostridium may help in the treatment of diseases such as cancer. Research shows that Clostridium can selectively target cancer cells. Some strains can enter and replicate within solid tumors. Clostridium could, therefore, be used to deliver therapeutic proteins to tumours. This use of Clostridium has been demonstrated in a variety of preclinical models.
- Mixtures of Clostridium species, such as Clostridium beijerinckii, Clostridium butyricum, and species from other genera have been shown to produce biohydrogen from yeast waste.
- Parte AC. "Clostridium". LPSN.
- Maczulak A (2011), "Clostridium", Encyclopedia of Microbiology, Facts on File, pp. 168–173, ISBN 978-0-8160-7364-1
- Hoffman B (2012). Williams gynecology (2nd ed.). New York: McGraw-Hill Medical. p. 65. ISBN 978-0071716727.
- Boutard M, Cerisy T, Nogue PY, Alberti A, Weissenbach J, Salanoubat M, Tolonen AC (November 2014). "Functional diversity of carbohydrate-active enzymes enabling a bacterium to ferment plant biomass". PLOS Genetics. 10 (11): e1004773. doi:10.1371/journal.pgen.1004773. PMC 4230839. PMID 25393313.
- Lopetuso LR, Scaldaferri F, Petito V, Gasbarrini A (August 2013). "Commensal Clostridia: leading players in the maintenance of gut homeostasis". Gut Pathogens. 5 (1): 23. doi:10.1186/1757-4749-5-23. PMC 3751348. PMID 23941657.
- UK Standards for Microbiology Investigations (October 10, 2011). "Identification of Clostridium Species". Standards Unit, Health Protection Agency. p. 7. 8. Retrieved November 3, 2013.
- Baron S, et al., eds. (1996). "Clostridia: Sporeforming Anaerobic Bacilli". Baron's Medical Microbiology (4th ed.). Univ. of Texas Medical Branch. ISBN 978-0-9631172-1-2.
- Kiu R, Brown J, Bedwell H, Leclaire C, Caim S, Pickard D, et al. (October 2019). "Clostridium perfringens strains and exploratory caecal microbiome investigation reveals key factors linked to poultry necrotic enteritis". Animal Microbiome. 1 (1): 12. doi:10.1186/s42523-019-0015-1. PMC 7000242. PMID 32021965.
- Kiu R, Hall LJ (August 2018). "An update on the human and animal enteric pathogen Clostridium perfringens". Emerging Microbes & Infections. 7 (1): 1–15. doi:10.1038/s41426-018-0144-8. PMC 6079034. PMID 30082713.
- Meites E, Zane S, Gould C (September 2010). "Fatal Clostridium sordellii infections after medical abortions". The New England Journal of Medicine. 363 (14): 1382–3. doi:10.1056/NEJMc1001014. PMID 20879895.
- Tortora GJ, Funke BR, Case CL (2010), Microbiology: An Introduction (10th ed.), Benjamin Cummings, pp. 87, 134, 433, ISBN 978-0-321-55007-1
- Maczulak A (2011), "stain", Encyclopedia of Microbiology, Facts on File, pp. 726–729, ISBN 978-0-8160-7364-1
- Willems H, Jäger C, Reiner G (2007), "Polymerase Chain Reaction", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–27, doi:10.1002/14356007.c21_c01.pub2, ISBN 978-3527306732
- Leikin JB, Paloucek FP, eds. (2008), "Clostridium perfringens Poisoning", Poisoning and Toxicology Handbook (4th ed.), Informa, pp. 892–893, ISBN 978-1-4200-4479-9
- Actor P, Chow AW, Dutko FJ, McKinlay MA (2007), "Chemotherapeutics", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–61, doi:10.1002/14356007.a06_173, ISBN 978-3527306732
- Harvey RA, ed. (2012), Lippincott's Illustrated Reviews: Pharmacology (5th ed.), Lippincott, pp. 389–404, ISBN 978-1-4511-1314-3
- Jelen P (2007), "Foods, 2. Food Technology", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–38, doi:10.1002/14356007.a11_523, ISBN 978-3527306732
- Burkhalter G, Steffen C, Puhan Z (2007), "Cheese, Processed Cheese, and Whey", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–11, doi:10.1002/14356007.a06_163, ISBN 978-3527306732
- Honikel K (2007), "Meat and Meat Products", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–17, doi:10.1002/14356007.e16_e02.pub2, ISBN 978-3527306732
- Samel Ul, Kohler W, Gamer AO, Keuser U (2007), "Propionic Acid and Derivatives", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–18, doi:10.1002/14356007.a22_223, ISBN 978-3527306732
- Zink R, Pfeifer A (2007), "Health Value Added Foods", Ullmann's Encyclopedia of Industrial Chemistry (7th ed.), Wiley, pp. 1–12, doi:10.1002/14356007.d12_d01, ISBN 978-3527306732
- Newman G (1904), Bacteriology and the Public Health, P. Blakiston's Son and Company.
- Velickovic M, Benabou R, Brin MF (2001). "Cervical dystonia pathophysiology and treatment options". Drugs. 61 (13): 1921–43. doi:10.2165/00003495-200161130-00004. PMID 11708764. S2CID 46954613.
- Doherty GM, ed. (2005), "Inflammation, Infection, & Antimicrobial Therapy", Current Diagnosis & Treatment: Surgery, McGraw-Hill, ISBN 978-0-07-159087-7
- "Providing for a Sustainable Energy Future". Bioengineering Resources, inc. Retrieved 21 May 2007.
- Saint-Amans S, Perlot P, Goma G, Soucaille P (August 1994). "High production of 1,3-propanediol from gycerol by clostridium butyricum VPI 3266 in a simply controlled fed-batch system". Biotechnology Letters. 16 (8): 831–836. doi:10.1007/BF00133962. S2CID 2896050.
- Mengesha A, Dubois L, Paesmans K, Wouters B, Lambin P, Theys J (2009). "Clostridia in Anti-tumor Therapy". In Brüggemann H, Gottschalk G (eds.). Clostridia: Molecular Biology in the Post-genomic Era. Caister Academic Press. ISBN 978-1-904455-38-7.
- Chou CH, Han CL, Chang JJ, Lay JJ (October 2011). "Co-culture of Clostridium beijerinckii L9, Clostridium butyricum M1 and Bacillus thermoamylovorans B5 for converting yeast waste into hydrogen". International Journal of Hydrogen Energy. 36 (21): 13972–13983. doi:10.1016/j.ijhydene.2011.03.067.