The 38th National Assembly of Quebec was elected in the 2007 Quebec general election and sat from May 8, 2007 to November 5, 2008. Jean Charest (PLQ) was the Premier and Mario Dumont (ADQ) was the leader of the opposition. It ended when the 2008 general election was called.
Cabinet Ministers are in Bold, Leaders are in Italics and the President of the National Assembly has a Dagger next to his name.
- Rosaire Bertrand MNA for Charlevoix resigned on August 13, 2007, to make way for a by-election later that year in which the new Parti Québécois leader, Pauline Marois, would be a candidate. 
- Diane Lemieux, who was the PQ House Leader resigned as MNA for Bourget on October 17, 2007 and replaced as House Leader by François Gendron. 
- André Boisclair resigned as MNA for Pointe-aux-Trembles on November 15, 2007.
- Roch Cholette announced his resignation as MNA for Hull on April 3, 2008 taking effect on April 9, 2008.
- Maryse Gaudreault for the Quebec Liberal Party was elected in Hull and Maka Kotto and Nicole Léger of the Parti Québécois were elected in Bourget and Pointe-aux-Trembles respectively during by-elections held on May 12, 2008. 
- Philippe Couillard resigned as MNA for Jean-Talon on June 25, 2008.
- Michel Bissonnet resigned as MNA for Jeanne-Mance-Viger on July 14, 2008.
- Yves Bolduc won the Jean-Talon by-election on September 29, 2008 with 58% of the popular vote. 
- Russell Copeman resigned as MNA for Notre-Dame-de-Grace on October 22, 2008. [permanent dead link]
- André Riedl, MNA for Iberville, and Pierre-Michel Auger, MNA for Champlain crossed the floor from the ADQ to the Liberals on October 23, 2008. 
- Premier and Executive Council President: Jean Charest
- Deputy Premier: Nathalie Normandeau
- Agriculture, Fisheries and Food: Laurent Lessard
- Employment and Social Solidarity: Sam Hamad
- Labor: David Whissell
- Government Administration, Government Services and President of the Treasury Board: Monique Jérôme-Forget
- Information Access:Benoît Pelletier
- Culture, Communications and Status of Women: Christine St-Pierre
- International Relations: Monique Gagnon-Tremblay
- Indian Affairs: Benoît Pelletier
- Canadian Francophonie: Benoît Pelletier
- Health and Social Services: Philippe Couillard (2007-2008), Yves Bolduc (2008–present)
- Education: Michelle Courchesne
- Immigration and Cultural Communities: Yolande James
- Seniors: Marguerite Blais
- Family: Michelle Courchesne
- Transportation: Julie Boulet
- Canadian Intergovernmental Affairs: Benoît Pelletier
- Municipal Affairs and Regions: Nathalie Normandeau
- Democratic Institutions Reform: Benoît Pelletier
- Recreation and Sport: Michelle Courchesne
- Sustainable Development, Environment and Parks: Line Beauchamp
- Natural Resources and Wildlife: Claude Bechard
- Justice: Jacques P. Dupuis
- Public Safety: Jacques P. Dupuis
- Finances: Monique Jerome-Forget
- Revenue: Jean-Marc Fournier
- Tourism: Raymond Bachand
- Economic Development, Innovation and Export Trade : Raymond Bachand