Incumbent Federalist William Bingham, who was elected in 1795, was not a candidate for re-election to another term. The Pennsylvania General Assembly, consisting of the House of Representatives and the Senate, convened on February 19, 1801, to elect a new Senator to fill the term beginning on March 4, 1801. Two ballots were recorded. The results of the second and final ballot of both houses combined are as follows:
- "U.S. Senate Election - 19 February 1801" (PDF). Wilkes University. Retrieved 28 September 2013.
| Pennsylvania U.S. Senate election (Class III)